MABT81 | Anti-JAM-C Antibody, clone H33 (azide free)

MABT81
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MFC, ICC, FUNCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT81
      DescriptionAnti-JAM-C Antibody, clone H33 (azide free)
      Alternate Names
      • Junctional adhesion molecule C
      • JAM-C
      • JAM-2
      • Junctional adhesion molecule 3
      • JAM-3
      Background InformationJunctional adhesion molecule C (JAM-C) is an adhesion molecule found at endothelial intracellular junctions that plays a role in leukocyte transendothelial migration and other inflammatory events. JAM-C seems to be important in the pathophysiology of pancreatitis and therefore could be a promising clinical target. JAM-C expression has been shown to be increased when T lymphocytes are activated. Research has shown that controlling JAM-C may also be a way of controlling pathological angiogenesis.
      References
      Product Information
      FormatPurified
      Control
      • VEGF treated HUVEC cells
      PresentationPurified rat monoclonal IgG2aκ in PBS without 0.05% sodium azide (preservative free).
      Applications
      ApplicationAnti-JAM-C Antibody, clone H33 (azide free) is an antibody against JAM-C for use in FC & IC.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Affects Function
      Application NotesInhibition of Activity Assay: A representative lot was used by an independent laboratory to inhibit microvessel outgrowth in an adapted mouse aortic ring assay (Lamagna, C., et al. (2005). Cancer Res. 65:5703-5710).
      Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC (Lamagna, C., et al. (2005). Cancer Res. 65:5703-5710).
      Functional Blocking: Mice treated with a representative lot of this antibody showed a trend toward a reduced number of leukocytes in the tissue at every time point analyzed (Bradfield, P.F., et al. (2007). Blood, 110(7):2545-2555).
      Functional Blocking: Mice treated with a representative lot of this antibody showed a trend toward reduced diabetes incidence and blood glucose over time (Christen, S., et al. PLoS ONE. 8(1):e54675)
      Biological Information
      ImmunogenFull length recombinant protein corresponding to mouse JAM-C.
      CloneH33
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human.
      Predicted to react with Mouse based immunogen design.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Jam3
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: May participate in cell-cell adhesion distinct from tight junctions.

      SUBUNIT STRUCTURE: Interacts with JAM2 (By similarity).

      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (Potential).

      TISSUE SPECIFICTY: Endothelial cells.

      SEQUENCE SIMILARITIES: Belongs to the immunoglobulin superfamily.

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
      Molecular Weight35 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in VEGF treated HUVEC cells.

      Flow Cytometry Analysis: A 1:125 dilution of this antibody detected JAM-C in VEGF treated HUVEC cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-JAM-C, clone H33 (azide free) - 2074808 2074808
      Anti-JAM-C, clone H33 (azide free) - 2147107 2147107
      Anti-JAM-C, clone H33 (azide free) - 2193453 2193453
      Anti-JAM-C, clone H33 (azide free) - NRG1868654 NRG1868654

      References

      Reference overviewApplicationSpeciesPub Med ID
      Blocking Junctional Adhesion Molecule C Enhances Dendritic Cell Migration and Boosts the Immune Responses against Leishmania major.
      Ballet, R; Emre, Y; Jemelin, S; Charmoy, M; Tacchini-Cottier, F; Imhof, BA
      PLoS pathogens  10  e1004550  2014

      Show Abstract
      25474593 25474593
      Blockade but Not Overexpression of the Junctional Adhesion Molecule C Influences Virus-Induced Type 1 Diabetes in Mice.
      Christen, Selina, et al.
      PLoS ONE, 8: e54675 (2013)  2013

      Show Abstract
      Neutralization AssayMouse23372751 23372751
      JAM-C regulates unidirectional monocyte transendothelial migration in inflammation.
      Bradfield, Paul F, et al.
      Blood, 110: 2545-55 (2007)  2007

      Show Abstract
      Blocking AssayMouse17625065 17625065
      Antibody against junctional adhesion molecule-C inhibits angiogenesis and tumor growth.
      Lamagna, C; Hodivala-Dilke, KM; Imhof, BA; Aurrand-Lions, M
      Cancer research  65  5703-10  2005

      Show Abstract
      15994945 15994945
      Dual interaction of JAM-C with JAM-B and alpha(M)beta2 integrin: function in junctional complexes and leukocyte adhesion.
      Lamagna, C; Meda, P; Mandicourt, G; Brown, J; Gilbert, RJ; Jones, EY; Kiefer, F; Ruga, P; Imhof, BA; Aurrand-Lions, M
      Molecular biology of the cell  16  4992-5003  2005

      Show Abstract
      16093349 16093349
      JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells.
      Aurrand-Lions, M; Duncan, L; Ballestrem, C; Imhof, BA
      The Journal of biological chemistry  276  2733-41  2001

      Show Abstract
      11053409 11053409

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies