ABE203 | Anti-JARID1D Antibody

ABE203
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE203
      DescriptionAnti-JARID1D Antibody
      Alternate Names
      • Lysine-specific demethylase 5D
      • Histocompatibility Y antigen
      • H-Y
      • Histone demethylase JARID1D
      • Jumonji/ARID domain-containing protein 1D
      • Protein SmcY
      Background InformationAlso known as Lysine-specific demethylase 5D, this histone demethylase is specific for 'Lys-4' of histone H3 and is known to play a central role in histone function and spermatogenesis. It demethylates trimethylated and dimethylated, but not monomethylated H3 'Lys-4'. Uniprot.org states two different isoforms of this enzyme.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human testis tissue lysate.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-JARID1D Antibody (rabbit polyclonal antibody) validated in WB, ICC, IP to detect JARID1D also known as Lysine-specific demethylase 5D, Histocompatibility Y antigen, H-Y, Histone demethylase JARID1D.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: 2 µg/mL from a representative lot detected JARID1D in mESCs.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated JARID1D in human testis tissue lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human JARID1D.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein containing zinc finger domains. A short peptide derived from this protein is a minor histocompatibility antigen which can lead to graft rejection of male donor cells in a female recipient. Alternative splicing results in multiple transcript variants.
      Gene Symbol
      • KDM5D
      • HY
      • HYA
      • JARID1D
      • KIAA0234
      • SMCY
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May play a role in spermatogenesis.

      COFACTOR: Ascorbate. Binds 1 Fe2+ ion per subunit.

      SUBUNIT STRUCTURE: Interacts with PCGF6 and MSH5.

      SUBCELLULAR LOCATION: Nucleus.
      Molecular Weight~174 kDa observed. Two isoforms may be observed due to alternative splicing at a molecular weight of ~167 kDa and ~174 kDa in some cell lysates. An uncharacterized band at a molecular weight of ~255 kDa may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human testis tissue lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected JARID1D in 10 µg of human testis tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies