09-816 | Anti-JMJD1B Antibody

09-816
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Rb, Rhesus Macaque, EqWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-816
      DescriptionAnti-JMJD1B Antibody
      Alternate Names
      • JMJD
      • Jumonji domain-containing protein 1B
      • Nuclear protein 5qNCA
      • chromosome 5 open reading frame 7
      • jumonji domain containing 1B
      Background InformationMethylation of histones plays a critical role in regulating chromatin structure and transcription. The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JHDM2A, JMJD1B/JHDM2B, and JMJD1C/JHDM2C. JMJD1B has been shown to specifically demethylate mono-methyl and di-methyl histone H3 on lysine9. H3 Lys 9 methylation is a highly studied histone mark that represses gene expression and implicates JMJD1B as a key regulator of heterochromatin assembly. Deletions in the gene encoding JMJD1B are frequently associated with myeloid leukemias and myelodysplasias, suggesting JMJD1B also may act as a tumor suppressor.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa nuclear extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-JMJD1B Antibody is a rabbit polyclonal antibody for detection of JMJD1B also known as Jumonji domain-containing protein 1B, Nuclear protein 5qNCA, chromosome 5 open reading frame 7 & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected JMJD1B in HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human JMJD1B.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Rhesus Macaque
      • Horse
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Rabbit, Rhesus Macaque, and Equine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • 5qNCA
      • JHDM2B
      • KDM3B
      • JMJD1B
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.

      COFACTOR: Binds 1 Fe2+ ion per subunit (By similarity).

      SUBCELLULAR LOCATION: Nucleus (By similarity).

      TISSUE SPECIFICTY: Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver.

      DOMAIN: Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors (By similarity).

      MISCELLANEOUS: Its gene is located in the 5q region of the genome which is deleted in del(5q) interstitial deletion, a frequent deletion found in myeloid leukemias and myelodysplasias, suggesting that it may be a good candidate for the del(5q) tumor suppressor gene.

      SEQUENCE SIMILARITIES: Belongs to the JHDM2 histone demethylase family.

      Contains 1 JmjC domain.

      SEQUENCE CAUTION: The sequence BAA83034.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
      Molecular Weight~260 kDa observed. Uncharacterized bands may be observed in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

      Western Blot Analysis: 1 µg/mL of this antibody detected JMJD1B on 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies