04-1130 | Anti-LEF-1/TCF-1 α Antibody, clone EP2030Y, rabbit monoclonal

04-1130
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IHC, FCRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1130
      DescriptionAnti-LEF-1/TCF-1 α Antibody, clone EP2030Y, rabbit monoclonal
      Alternate Names
      • lymphoid enhancer-binding factor 1
      • T cell-specific transcription factor 1-alpha
      Background InformationLymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate, and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants.
      References
      Product Information
      FormatUnpurified
      Control
      • Jurkat cell lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected LEF-1/TCF-1 α in human colon adenocarcinoma tissue.
      Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot was used in IC.
      Flow Cytometry: A 1:20 dilution of a representative lot was used in flow cytometry.
      Biological Information
      ImmunogenSynthetic peptide corresponding to human LEF-1/TCF-1 α.
      CloneEP2030Y
      HostRabbit
      SpecificityThis antibody recognizes LEF-1/TCF-1 α. Recognition mpas to an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LEF-1
      • TCF1ALPHA
      • TCF1-alpha
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~55 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Jurkat cell lysates.
      Western Blot Analysis: A 1:25,000-50,000 dilution of this antibody was used to detect LEF-1/TCF-1 α in Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies