MABF471 | Anti-Ly-6G Antibody (mouse), PerCP-Cy5.5, clone 1A8

MABF471
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFCRPerCP-Cy5.5Monoclonal Antibody
      Description
      Catalogue NumberMABF471
      DescriptionAnti-Ly-6G Antibody (mouse), PerCP-Cy5.5, clone 1A8
      Alternate Names
      • Lymphocyte antigen 6G
      • Ly-6G
      • Ly-6G.1
      Background InformationThe 1A8 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the 1A8 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: for identification of Ly-C, an alternative antibody, clone RB6-8C5, has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C.
      References
      Product Information
      FormatPerCP-Cy5.5
      PresentationPurified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
      Applications
      ApplicationThis rat monoclonal antibody Anti-Ly-6G (mouse), PerCP-Cy5.5 & clone 1A8 validated for use in Flow Cytometry for the detection of Ly-6G.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenImmunogen information for this antibody is proprietary information.
      Clone1A8
      Concentration0.2 mg/mL
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Ly6g
      Purification MethodSize Exclusion Chromatography
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

      Flow Cytometry Analysis: 0.25 µg of this antibody detected Ly-6G in one million C57Bl/6 bone marrow cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications