04-383 | Anti-MKK6 NT Antibody, rabbit monoclonal

04-383
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-383
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-MKK6 NT Antibody, rabbit monoclonal
      Alternate Names
      • MAPKK6
      • MEK6
      • MKK6
      • PRKMK6
      • SAPKK3
      References
      Product Information
      FormatPurified
      Control
      • POSITIVE: HeLa cells.
      PresentationRabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
      Applications
      ApplicationThis Anti-MKK6 NT Antibody is validated for use in IP, WB for the detection of MKK6 NT.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blot: 1:10,000.
      Immunoprecipitation: 1:50. The use of protein G agarose is recommended.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to residues in the N-term of human MKK6.
      EpitopeN-terminus
      HostRabbit
      SpecificityRecognizes MKK6.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
      Gene Symbol
      • MAP2K6
      • MEK6
      • PRKMK6
      • SAPKK3
      • MAPKK6
      • MKK6
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P52564 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
      SIZE: 334 amino acids; 37492 Da
      SUBUNIT: Interacts with Yersinia yopJ.
      TISSUE SPECIFICITY: Isoform 2 is only expressed in skeletal muscle. Isoform 1, on the other hand, is found in skeletal muscle, heart, and in lesser extent in liver or pancreas.
      PTM: Weakly autophosphorylated. & Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
      SIMILARITY: SwissProt: P52564 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight36 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot analysis.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-MKK6 NT, rabbit monoclonal - 0611045814 0611045814
      Anti-MKK6 NT, rabbit monoclonal - JBC1371289 JBC1371289
      anti-MKK6 NT, rabbit monoclonal - NG1915291 NG1915291

      References

      Reference overviewPub Med ID
      Active kinase proteome screening reveals novel signal complexity in cardiomyopathy
      Fernando, Pasan, et al
      Mol Cell Proteomics, 4:673-82 (2005)  2005

      15722372 15722372
      Regulation of p38 MAPK by MAPK kinases 3 and 6 in fibroblast-like synoviocytes.
      Inoue, Tomoyuki, et al.
      J. Immunol., 174: 4301-6 (2005)  2005

      Show Abstract
      15778394 15778394
      p38 Mitogen-activated protein kinase-dependent and -independent signaling of mRNA stability of AU-rich element-containing transcripts
      Frevel, Mathias A E, et al
      Mol Cell Biol, 23:425-36 (2003)  2003

      12509443 12509443

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      MABS810 Anti-MAP2K6, clone 3H12C8 Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies