AB6070 | Anti-MT1-MMP Antibody, hemopexin domain

AB6070
50 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Eq, Chp, Rhesus MacaqueWB, IH(P)RbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB6070
      ReplacesAB8104
      DescriptionAnti-MT1-MMP Antibody, hemopexin domain
      Alternate Names
      • Matrix metalloproteinase-14
      • MMP-14
      • MMP-X1
      • Membrane-type matrix metalloproteinase 1
      • MT-MMP 1
      • MTMMP1
      • Membrane-type-1 matrix metalloproteinase
      • MT1-MMP
      • MT1MMP
      Background InformationMatrix metalloproteinases (MMPs) are a family of secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes degrade components of extracellular matrix (ECM), including collagens, fibronectin, laminin and other glycoproteins. MMPs play an important role in wound healing, apoptosis, development, angiogenesis, cancer metastases, and tissue remodeling. Most MMPs are secreted as inactive proproteins which are cleaved and activated; however, MT1-MMP (MMP-14) is a member of the membrane-type subfamily. Each member of this subfamily has a transmembrane domain suggesting these proteins are expressed at the cell’s surface rather than secreted. MT1-MMP is capable of mediating pericellular proteolysis of ECM components and thought to be an important tool for cellular remodeling of the ECM. It is also involved in endothelial cell migration, and although not fully characterized, MT1-MMP activity appears to modulate endothelial invasion, and formation of capillary tubes during angiogenesis. It also appears to be a factor in monocyte recruitment during inflammation. MT1-MMP also activates MMP-2 protein, an activity involved in tumor invasion.
      References
      Product Information
      FormatPurified
      Control
      • HT-1080 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-MT1-MMP Antibody, hemopexin domain is validated for use in WB, IH(P) for the detection of MT1-MMP.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:400 dilution of this antibody detected MT1-MMP in human and rat colon tissue.
      Biological Information
      ImmunogenRecombinant protein corresponding to the entire hemopexin-like domain of MT1-MMP.
      EpitopeHemopoxin Domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the hemopoxin domain of MT1-MMP.
      Species Reactivity
      • Human
      • Rat
      • Horse
      • Chimpanzee
      • Rhesus Macaque
      Species Reactivity NoteDemonstrated to react with Human and Rat.
      Predicted to react with Horse, Chimpanzee and Rhesus Macaque based on 100% sequence homology. Other homologies: Pig, Bovine, and Canine (98% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySummary: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq].
      Gene Symbol
      • MMP14
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.

      CATALYTIC ACTIVITY: Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.

      COFACTOR: Binds 1 zinc ion per subunit (by similarity). Calcium (by similarity).

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (potential). Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

      TISSUE SPECIFICITY: Expressed in stromal cells of colon, breast, and head and neck.

      DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

      POST-TRANSLATIONAL MODIFICATION: The precursor is cleaved by a furin endopeptidase (by similarity).

      SEQUENCE SIMILARITIES: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
      Molecular Weight~66 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HT-1080 cell lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected MT1-MMP in 10 µg of HT-1080 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies