MAB1435 | Anti-Macrophages/Monocytes Antibody, clone ED-1

MAB1435
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RFC, IHC, IP, RIA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1435
      ReplacesCBL1511; CBL260
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Macrophages/Monocytes Antibody, clone ED-1
      Background InformationMonocytes are produced by the bone marrow from haematopoietic stem cell precursors called monoblasts. Monocytes circulate in the bloodstream for about one to three days and then typically move into tissues throughout the body. They consist of between 3 to 8 percent of the leukocytes in the blood. In the tissues monocytes mature into different types of macrophages at different anatomical locations.
      Macrophages are a type of white blood cell derived from monocytes that engulf invading antigenic molecules, viruses, and microorganisms and then display fragments of the antigen to activate helper T cells; ultimately stimulating the production of antibodies against the antigen.
      References
      Product Information
      FormatPurified
      Control
      • Macrophages, monocytes, lymphoid organs
      PresentationPurified mouse IgG1 in PBS (pH 7.4) without sodium azide
      Applications
      ApplicationThis Anti-Macrophages/Monocytes Antibody, clone ED-1 is validated for use in Flow Cytometry (FC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Radioimmunoassay (RIA), and Western Blotting (WB) for the detection of CD68.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Immunoprecipitation
      • Radioimmunoassay
      • Western Blotting
      Application NotesImmunohistology on frozen sections at 1:50-1:300. Paraffin embedded tissue (Bouin fixation): 1:50-1:300 (requires pretreatment with trypsin or pronase).

      Flow cytometry at 1:100 - 1:200. Use 10 μL of this working dilution to label 10E6 cells.

      Radioimmunoassay

      Also suitable for Immunoprecipitation, and Western Blotting.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRat spleen cells
      CloneED-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes a single chain glycoprotein of 90-100 kD that is expressed on the lysomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen has many characteristics in common with macrosialin in the mouse and CD68 in the human.
      Clone ED1, which recognises a CD68 like molecule in rat is an excellent marker of activated microglia. For pan microglial markers, staining both resting and activated microglia, use like CD11b (MAB1387Z and others) or iba-1 (MABN92).
      IsotypeIgG1
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A purified.
      Molecular Weight90-100 kD
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] MONOCLONAL ANTIBODY - 2121732 2121732
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] MONOCLONAL ANTIBODY - 2426502 2426502
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] MONOCLONAL ANTIBODY - 2449204 2449204
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] -2552332 2552332
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] -2626052 2626052
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] -2665054 2665054
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] -2689143 2689143
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] -2716532 2716532
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] -2716532A 2716532A
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] -2718364 2718364

      References

      Reference overviewApplicationSpeciesPub Med ID
      Neuregulin-1 inhibits neuroinflammatory responses in a rat model of organophosphate-nerve agent-induced delayed neuronal injury.
      Li, Y; Lein, PJ; Ford, GD; Liu, C; Stovall, KC; White, TE; Bruun, DA; Tewolde, T; Gates, AS; Distel, TJ; Surles-Zeigler, MC; Ford, BD
      Journal of neuroinflammation  12  64  2015

      Show Abstract
      25880399 25880399
      Feasibility and safety of continuous and chronic bilateral deep brain stimulation of the medial forebrain bundle in the naïve Sprague-Dawley rat.
      Furlanetti, LL; Döbrössy, MD; Aranda, IA; Coenen, VA
      Behavioural neurology  2015  256196  2015

      Show Abstract
      25960609 25960609
      Distinctive tooth-extraction socket healing: bisphosphonate versus parathyroid hormone therapy.
      Kuroshima, S; Mecano, RB; Tanoue, R; Koi, K; Yamashita, J
      Journal of periodontology  85  24-33  2014

      Show Abstract
      23688101 23688101
      Transient but not permanent benefit of neuronal progenitor cell therapy after traumatic brain injury: potential causes and translational consequences.
      Skardelly, M; Gaber, K; Burdack, S; Scheidt, F; Schuhmann, MU; Hilbig, H; Meixensberger, J; Boltze, J
      Frontiers in cellular neuroscience  8  318  2014

      Show Abstract
      25352780 25352780
      Abiotic-biotic characterization of Pt/Ir microelectrode arrays in chronic implants.
      Prasad, A; Xue, QS; Dieme, R; Sankar, V; Mayrand, RC; Nishida, T; Streit, WJ; Sanchez, JC
      Frontiers in neuroengineering  7  2  2014

      Show Abstract
      24550823 24550823
      PDGF is required for remyelination-promoting IgM stimulation of oligodendrocyte progenitor cell proliferation.
      Watzlawik, JO; Warrington, AE; Rodriguez, M
      PloS one  8  e55149  2013

      Show Abstract
      23383310 23383310
      Chemerin connects fat to arterial contraction.
      Watts, SW; Dorrance, AM; Penfold, ME; Rourke, JL; Sinal, CJ; Seitz, B; Sullivan, TJ; Charvat, TT; Thompson, JM; Burnett, R; Fink, GD
      Arteriosclerosis, thrombosis, and vascular biology  33  1320-8  2013

      Show Abstract
      23559624 23559624
      The use of poly(N-[2-hydroxypropyl]-methacrylamide) hydrogel to repair a T10 spinal cord hemisection in rat: a behavioural, electrophysiological and anatomical examination.
      Pertici, V; Amendola, J; Laurin, J; Gigmes, D; Madaschi, L; Carelli, S; Marqueste, T; Gorio, A; Decherchi, P
      ASN neuro  5  149-66  2013

      Show Abstract
      23614684 23614684
      Immunohistochemical, ultrastructural and functional analysis of axonal regeneration through peripheral nerve grafts containing Schwann cells expressing BDNF, CNTF or NT3.
      Godinho, MJ; Teh, L; Pollett, MA; Goodman, D; Hodgetts, SI; Sweetman, I; Walters, M; Verhaagen, J; Plant, GW; Harvey, AR
      PloS one  8  e69987  2013

      Show Abstract
      23950907 23950907
      Gene expression patterns following unilateral traumatic brain injury reveals a local pro-inflammatory and remote anti-inflammatory response.
      White, TE; Ford, GD; Surles-Zeigler, MC; Gates, AS; Laplaca, MC; Ford, BD
      BMC genomics  14  282  2013

      Show Abstract
      23617241 23617241