MAB363 | Anti-NMDAR1 Antibody, clone 54.1

MAB363
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Mk, R, XnELISA, ICC, IHC, IH(P), RIAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB363
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-NMDAR1 Antibody, clone 54.1
      Alternate Names
      • N-methyl-D-aspartate receptor channel, subunit zeta-1
      • N-methyl-D-aspartate receptor subunit NR1
      • NMDA receptor 1
      • glutamate [NMDA] receptor subunit zeta 1
      • glutamate receptor, ionotropic
      • N-methyl D-aspartate 1
      Background InformationGlutamate is a widely utilized excitatory neurotransmitter in mammalian brain. The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate (KA) and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are referred to as non-NMDA receptors. Two distinct genes encode the NMDA receptor subunits, NR1 and NR2. The NR1 subunit is expressed in abundance in virtually all regions of the brain.
      References
      Product Information
      FormatPurified
      Control
      • Rat brain and spinal cord tissue
      PresentationPurified mouse monoclonal IgG2a liquid in buffer PBS, no preservative.
      Applications
      ApplicationAnti-NMDAR1 Antibody, clone 54.1 is an antibody against NMDAR1 for use in ELISA, IC, IH, IH(P), RIA.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Radioimmunoassay
      Application NotesImmunocytochemistry:
      A previous lot of this antibody was used in IC.

      RIA:
      A previous lot of this antibody was used in RIA.

      ELISA:
      A previous lot of this antibody was used in ELISA.

      Immunohistochemistry: 1:500-2000, on 2-4% paraformaldehyde fixed tissue: typically free-floating, frozen or vibratome sections 24-48 hours at 4°C. Paraffin embedded formalin fixed sections have also been used at similar dilutions and incubation times. Care should be taken not to over fix the tissues. The clone 54.1 has been used for both light and electron microscopy.

      Western blot:
      1:500 with 50 migrograms/lane of material. Recognizes a 103-116kDa band.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant fusion protein containing GST and NMDAR1 aa. 660-811.
      EpitopeExtracellular loop between transmembrane regions III & IV.
      Clone54.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes an epitope between amino acids 660-811 of the NMDAR1 receptor. Shows no cross-reactivity with NMDAR2, NMDAR3, NMDAR4 or NMDAR5.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Monkey
      • Rat
      • Xenopus
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.
      Gene Symbol
      • GRIN1
      • NR1
      • Glutamate
      • NMDAR1
      • NMDA1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q05586 # NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors (By similarity).
      SIZE: 938 amino acids; 105373 Da
      SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B, GRIN3A or GRIN3B and PPP2CB. Interacts with DLG4 and MPDZ (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Note=Enriched in post-synaptic plasma membrane and post-synaptic densities (By similarity).
      PTM: NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity (By similarity).
      SIMILARITY: SwissProt: Q05586 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
      Molecular Weight103-116 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by immunohistochemistry on rat brain and spinal cord tissue.

      Immunohistochemistry(paraffin) Analysis:
      NMDAR1 representative staining pattern/morphology on rat spinal cord at 1:200 dilution. Immunoreactivity is seen as integral membrane and nerve fiber staining of neurons. Frozen section. IHC -Frozen Staining Examples: Rat Spinal Cord
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NMDAR1, extracellular loop -2795092 2795092
      Anti-NMDAR1, extracellular loop between transmembrane regions III IV, clone 54.1 - 1986128 1986128
      Anti-NMDAR1, extracellular loop between transmembrane regions III IV, clone 54.1 - 2011450 2011450
      Anti-NMDAR1, extracellular loop between transmembrane regions III IV, clone 54.1 - 2041559 2041559
      Anti-NMDAR1, extracellular loop between transmembrane regions III IV, clone 54.1 - 2090096 2090096
      Anti-NMDAR1, extracellular loop between transmembrane regions III IV, clone 54.1 - 2219311 2219311
      Anti-NMDAR1, extracellular loop between transmembrane regions III IV, clone 54.1 - JC1668379 JC1668379
      Anti-NMDAR1, extracellular loop between transmembrane regions III IV, clone 54.1 - LV1543884 LV1543884
      Anti-NMDAR1, extracellular loop between transmembrane regions III IV, clone 54.1 - LV1583519 LV1583519
      Anti-NMDAR1, extracellular loop between transmembrane regions III IV, clone 54.1 - NG1719705 NG1719705

      References

      Reference overviewApplicationSpeciesPub Med ID
      Human N-methyl D-aspartate receptor antibodies alter memory and behaviour in mice.
      Planagumà, J; Leypoldt, F; Mannara, F; Gutiérrez-Cuesta, J; Martín-García, E; Aguilar, E; Titulaer, MJ; Petit-Pedrol, M; Jain, A; Balice-Gordon, R; Lakadamyali, M; Graus, F; Maldonado, R; Dalmau, J
      Brain : a journal of neurology  138  94-109  2015

      Show Abstract
      25392198 25392198
      Developmental attenuation of N-methyl-D-aspartate receptor subunit expression by microRNAs.
      Corbel, C; Hernandez, I; Wu, B; Kosik, KS
      Neural development  10  20  2015

      Show Abstract
      26381867 26381867
      A novel approach identifies the first transcriptome networks in bats: a new genetic model for vocal communication.
      Rodenas-Cuadrado, P; Chen, XS; Wiegrebe, L; Firzlaff, U; Vernes, SC
      BMC genomics  16  836  2015

      Show Abstract
      26490347 26490347
      Comparison of diagnostic accuracy of microscopy and flow cytometry in evaluating N-methyl-D-aspartate receptor antibodies in serum using a live cell-based assay.
      Ramberger, M; Peschl, P; Schanda, K; Irschick, R; Höftberger, R; Deisenhammer, F; Rostásy, K; Berger, T; Dalmau, J; Reindl, M
      PloS one  10  e0122037  2015

      Show Abstract
      25815887 25815887
      Mapping synapses by conjugate light-electron array tomography.
      Collman, F; Buchanan, J; Phend, KD; Micheva, KD; Weinberg, RJ; Smith, SJ
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  5792-807  2015

      Show Abstract
      25855189 25855189
      Synaptic and cognitive improvements by inhibition of 2-AG metabolism are through upregulation of microRNA-188-3p in a mouse model of Alzheimer's disease.
      Zhang, J; Hu, M; Teng, Z; Tang, YP; Chen, C
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  14919-33  2014

      Show Abstract
      25378159 25378159
      Differential Changes in Postsynaptic Density Proteins in Postmortem Huntington's Disease and Parkinson's Disease Human Brains.
      Fourie, C; Kim, E; Waldvogel, H; Wong, JM; McGregor, A; Faull, RL; Montgomery, JM
      Journal of neurodegenerative diseases  2014  938530  2014

      Show Abstract
      26317010 26317010
      MET receptor tyrosine kinase controls dendritic complexity, spine morphogenesis, and glutamatergic synapse maturation in the hippocampus.
      Qiu, S; Lu, Z; Levitt, P
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  16166-79  2014

      Show Abstract
      Immunohistochemistry25471559 25471559
      In vitro ischemia triggers a transcriptional response to down-regulate synaptic proteins in hippocampal neurons.
      Fernandes, J; Vieira, M; Carreto, L; Santos, MA; Duarte, CB; Carvalho, AL; Santos, AE
      PloS one  9  e99958  2014

      Show Abstract
      Western Blotting24960035 24960035
      An Immunological Approach to Increase the Brain's Resilience to Insults.
      Lin, EJ; Symes, CW; Townsend-Nicholson, A; Klugmann, M; Klugmann, CB; Lehnert, K; Fong, D; Young, D; During, MJ
      ISRN neuroscience  2014  103213  2014

      Show Abstract
      24967312 24967312

      Brochure

      Title
      Pathways and Biomarkers of Glutamatergic Synapse Flyer

      Data Sheet

      Title
      Anti-NMDAR1, extracellular loop between transmembrane regions III & IV, clone 54.1 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies