AB9372 | Anti-Necdin Antibody

AB9372
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB9372
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Necdin Antibody
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
      Applications
      ApplicationThis Anti-Necdin Antibody is validated for use in WB, IH for the detection of Necdin.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunoblotting: 0.5-1.0 μg/mL on mouse brain tissue homogenate. The antibody reacts with the ~43 kD Necdin protein. Additional bands at ~35 kD and ~60 kD may been seen depending on sample and antibody concentration used.

      Immunohistochemistry: 1:100 mouse cerebral cortex (second somatosensory cortex) using HIER (heat induced epitope retrieval). Recommend using citrate, pH 6.0.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide of human Necdin at the N-terminus.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Necdin.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteProven to react with human, mouse, and rat. Predicted to react with rhesus monkey based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons.
      Gene Symbol
      • NDN
      • HsT16328
      • necdin
      • Necdin.
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q99608 # Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle- promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences (By similarity).
      SIZE: 321 amino acids; 36086 Da
      SUBUNIT: Binds to the transactivation domains of E2F1 and p53. Binds also SV40 large T antigen and adenovirus E1A. Interacts with nucleobindin 1 and 2 (By similarity).
      SUBCELLULAR LOCATION: Perikaryon. Nucleus. Note=Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix.
      TISSUE SPECIFICITY: Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post- mitotic neurons.
      DISEASE: SwissProt: Q99608 # Defects in NDN may be a cause of the Prader-Willi syndrome (PWS) which is a contiguous gene syndrome resulting from inactivity of the paternal copies of a number of genes on 15q11, through deletion or disruption of these genes or maternal uniparental disomy 15. The PWS syndrome is characterized by muscular hypotonia, mental retardation, short stature, obesity, hypogonadotropic hypogonadism, and small hands and feet.
      SIMILARITY: SwissProt: Q99608 ## Contains 1 MAGE domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Necdin - 1978763 1978763
      Anti-Necdin - 2064293 2064293
      Anti-Necdin - 2296749 2296749
      Anti-Necdin - 2352389 2352389
      Anti-Necdin - 2492830 2492830

      References

      Reference overviewApplicationPub Med ID
      Putative tumour suppressor gene necdin is hypermethylated and mutated in human cancer.
      De Faveri, LE; Hurst, CD; Platt, FM; Taylor, CF; Roulson, JA; Sanchez-Carbayo, M; Knowles, MA; Chapman, EJ
      British journal of cancer  108  1368-77  2013

      Show Abstract
      Immunohistochemistry23549060 23549060

      Data Sheet

      Title
      Anti-Necdin - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies