MABN140 | Anti-NeuN (rabbit monoclonal) Antibody, clone 27-4

MABN140
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RICC, WB, IHCRbPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN140
      DescriptionAnti-NeuN (rabbit monoclonal) Antibody, clone 27-4
      Background InformationNeuN is a RNA-binding protein that belongs to the evolutionarily conserved Fox-1 family. It is mainly involved RNA splicing. It is found exclusively in the nuclei of neuronal cells, but is also present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although. Some neurons fail to recognize NeuN at all ages in certain cells. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
      References
      Product Information
      FormatPurified
      Control
      • Mouse brain nuclear extract
      PresentationPurified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-NeuN (rabbit monoclonal) Antibody, clone 27-4 detects level of NeuN (rabbit monoclonal) & has been published & validated for use in ICC, Western Blotting & IHC.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 cortex and hippocampus primary cultures.

      Immunohistochemistry Analysis: A 1:400 dilution of this antibody detected NeuN in mouse cerebellum and hippocampus tissues.
      Biological Information
      ImmunogenRecombinant protein corresponding to mouse NeuN.
      Clone27-4
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with mouse and rat. Predicted to react with human based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      Molecular Weight~46/48 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse brain nuclear extract.

      Western Blot Analysis: A 1:2,000 dilution of this antibody detected NeuN in 10 µg of mouse brain nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NeuN (rabbit monoclonal), clone 27-4 - 2324697 2324697
      Anti-NeuN (rabbit monoclonal), clone 27-4 - 2452171 2452171
      Anti-NeuN (rabbit monoclonal), clone 27-4 - 2153062 2153062
      Anti-NeuN (rabbit monoclonal), -2523222 2523222
      Anti-NeuN (rabbit monoclonal), -2575775 2575775
      Anti-NeuN (rabbit monoclonal), -2625391 2625391
      Anti-NeuN (rabbit monoclonal), -2686158 2686158
      Anti-NeuN (rabbit monoclonal), -2709668 2709668
      Anti-NeuN (rabbit monoclonal), -2793637 2793637
      Anti-NeuN (rabbit monoclonal), clone 27-4 - 2049260 2049260

      References

      Reference overviewPub Med ID
      Evaluation of the percentage of ganglion cells in the ganglion cell layer of the rodent retina.
      Schlamp, CL; Montgomery, AD; Mac Nair, CE; Schuart, C; Willmer, DJ; Nickells, RW
      Molecular vision  19  1387-96  2013

      Show Abstract
      23825918 23825918

      Brochure

      Title
      New Products: Volume 3, 2012

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies