ABE991 | Anti-NeuroD1 Antibody

ABE991
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RWB, ChIP, IHCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABE991
      DescriptionAnti-NeuroD1 Antibody
      Alternate Names
      • Neurogenic differentiation factor 1
      • NeuroD1
      • Beta-cell E-box transcriptional activator 2
      • Beta2
      Background InformationNeuroD1 is a transcriptional activator that associates with p300 transcription co-activator protein to promote the expression of many important differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. NeuroD1 is required for amacrine cell fate as well as dendrite morphogenesis and the maintenance of cerebellar cortex. Also via its associate with chromatin, NeuroD1 helps regulate the expression of many other key transcriptional regulators of neurogenesis as well. NeuroD1 is expressed in developing brain and pancreas as well as in differentiating neurons in both the CNS and PNS systems. Disruption of NeuroD1 leads to early death in mice with several developmental and functional defects throughout the body pointing to this factors key role in development in many systems.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-NeuroD1 Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and Immunohistochemistry for the detection of NeuroD1.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A representative lot detected NeuroD1 in developing pituitary tissue (Lavoie, P., et al. (2008). Molecular Endocrinology. 22:1647-1657).
      Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated NeuroD1 in AtT-20 cell lysate (Langlais, D., et al. (2011). Molecular Endocrinology. 25:348-359).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse NeuroD1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Neurod1
      • Neurod
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~40 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NeuroD1 in 10 µg of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NeuroD1 - Q2448648 Q2448648

      References

      Reference overviewPub Med ID
      A pituitary-specific enhancer of the POMC gene with preferential activity in corticotrope cells.
      Langlais, D; Couture, C; Sylvain-Drolet, G; Drouin, J
      Molecular endocrinology (Baltimore, Md.)  25  348-59  2011

      Show Abstract
      21193556 21193556
      Developmental dependence on NurRE and EboxNeuro for expression of pituitary proopiomelanocortin.
      Lavoie, Pierre-Luc, et al.
      Mol. Endocrinol., 22: 1647-57 (2008)  2008

      Show Abstract
      18388149 18388149