ABN76 | Anti-Neurofilament H (200 kDa) Antibody

ABN76
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, B, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN76
      ReplacesAB1982
      DescriptionAnti-Neurofilament H (200 kDa) Antibody
      Alternate Names
      • Neurofilament heavy polypeptide
      • Neurofilament H
      Background InformationNeurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas, adrenal and extra-adrenal pheochromocytomas, carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung. For moreinformation, see the Nervous System Cell Type Specific Marker chart on the Millipore website.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Bovine cerebellum tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Neurofilament H (200 kDa) Antibody is validated for use in WB, IH(P) for the detection of Neurofilament H (200 kDa).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1 µg/mL dilution from a representative lot detected Neurofilament H in normal rat cerebellum tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to bovine Neurofilament H.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Bovine
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNeurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.
      Gene Symbol
      • NEFH
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

      POST-TRANSLATION MODIFICATION: Modification There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function. Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.

      POLYMORPHISM: The number of repeats is shown to vary between 29 and 30.

      INVOLVEMENT IN DISEASE: Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.

      SEQUENCE SIMILARITIES: Belongs to the intermediate filament family.

      SEQUENCE CAUTION: The sequence BAA74868.2 differs from that shown. Reason: Erroneous initiation. The sequence BAG63896.1 differs from that shown. Reason: Erroneous initiation.



      Molecular Weight~200 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in bovine cerebellum tissue lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected Neurofilament H in bovine cerebellum tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Neurofilament H (200 kDa) - 2458003 2458003
      Anti-Neurofilament H (200 kDa) - 1966930 1966930
      Anti-Neurofilament H (200 kDa) - 2211976 2211976
      Anti-Neurofilament H (200 kDa) - 2275638 2275638
      Anti-Neurofilament H (200 kDa) - 2290993 2290993
      Anti-Neurofilament H (200 kDa) - NRG1903979 NRG1903979
      Anti-Neurofilament H (200 kDa) -2550055 2550055
      Anti-Neurofilament H (200 kDa) -2584433 2584433
      Anti-Neurofilament H (200 kDa) -2601743 2601743
      Anti-Neurofilament H (200 kDa) -2650899 2650899

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies