07-1234 | Anti-Notch 2 Antibody, NT

07-1234
200 µL  
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      Overview

      Replacement Information
      Replacement Information07-1234 is a recommended replacement for part AB5713

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MELISA, WB, IH(P)RbSerumPolyclonal Antibody
      Description
      Catalogue Number07-1234
      ReplacesPART AB5713
      DescriptionAnti-Notch 2 Antibody, NT
      Alternate Names
      • Neurogenic locus notch homolog protein 2 precursor
      • Notch (Drosophila) homolog
      • Notch 2
      • Notch homolog 2 (Drosophila)
      Background InformationNotch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.
      References
      Product Information
      FormatSerum
      Control
      • NIH/3T3 Cell Lysate
      PresentationRabbit polyclonal IgG serum in buffer containing 0.02 M Potassium phosphate buffer with 0.15 M NaCl and 0.1% sodium azide
      Applications
      ApplicationAnti-Notch 2 Antibody, N-Terminus detects level of Notch 2 & has been published & validated for use in ELISA, WB, IH(P).
      Key Applications
      • ELISA
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesELISA:
      1:30,000-1:90,000 dilution from a previous lot was used in a standard sandwich ELISA assay against the peptide immunogen.
      Optimal working dilutions must be determined by the end user.

      Immunohistochemistry(paraffin):
      Representative testing from a previous lot.
      Optimal Staining of NOTCH-2 Polyclonal Antibody: Squamous Cell Carcinoma
      Biological Information
      ImmunogenSynthetic peptide from the N-terminal sequence of the cleaved N intracellular domain (NICD) human Notch 2.
      EpitopeCleaved N-terminus
      HostRabbit
      SpecificityNotch 2, cleaved N terminal. This epitope is exposed only after gamma secretase cleavage and is not accessible in the uncleaved form.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman and mouse. The antibody is also expected to react with rat due to immunogen sequence homology (14/15). Other species have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development.
      Gene Symbol
      • NOTCH2
      • hN2
      • OTTHUMP00000059536
      • AGS2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q04721 # Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity).
      SIZE: 2471 amino acids; 265405 Da
      SUBUNIT: Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds (By similarity). Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH2.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Notch 2 intracellular domain: Nucleus. Note=Following proteolytical processing NICD is translocated to the nucleus.
      TISSUE SPECIFICITY: Expressed in the brain, heart, kidney, lung, skeletal muscle and liver.
      PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C- terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). & Phosphorylated (By similarity).
      DISEASE: SwissProt: Q04721 # Defects in NOTCH2 are the cause of Alagille syndrome type 2 (ALGS2) [MIM:610205]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
      SIMILARITY: SwissProt: Q04721 ## Belongs to the NOTCH family. & Contains 6 ANK repeats. & Contains 35 EGF-like domains. & Contains 3 LNR (Lin/Notch) repeats.
      Molecular Weight~72 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on NIH/3T3 lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected cleaved NOTCH2 on 10 μg of NIH/3T3 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20°C in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Notch2, cleaved N-terminus 2458988
      Anti-Notch2, cleaved N-terminus - 2376420 2376420
      Anti-Notch2, cleaved N-terminus - 2492086 2492086
      Anti-Notch2, cleaved N-terminus - 1956744 1956744
      Anti-Notch2, cleaved N-terminus - 1986171 1986171
      Anti-Notch2, cleaved N-terminus - 2005399 2005399
      Anti-Notch2, cleaved N-terminus - 2049818 2049818
      Anti-Notch2, cleaved N-terminus - 2137048 2137048
      Anti-Notch2, cleaved N-terminus - 2224459 2224459
      Anti-Notch2, cleaved N-terminus - 2519220 2519220

      References

      Reference overviewApplicationPub Med ID
      Midkine promotes neuroblastoma through Notch2 signaling.
      Kishida, S; Mu, P; Miyakawa, S; Fujiwara, M; Abe, T; Sakamoto, K; Onishi, A; Nakamura, Y; Kadomatsu, K
      Cancer research  73  1318-27  2013

      Show Abstract
      Immunohistochemistry23243020 23243020
      Notch signaling components are upregulated during both endochondral and intramembranous bone regeneration.
      Dishowitz, MI; Terkhorn, SP; Bostic, SA; Hankenson, KD
      Journal of orthopaedic research : official publication of the Orthopaedic Research Society  30  296-303  2012

      Show Abstract
      Immunohistochemistry (Paraffin)21818769 21818769

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies