MABE1044 | Anti-PARP10/ARTD10 Antibody, clone 5H11

MABE1044
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1044
      DescriptionAnti-PARP10/ARTD10 Antibody, clone 5H11
      Alternate Names
      • Poly [ADP-ribose] polymerase 10
      • PARP-10
      • ADP-ribosyltransferase diphtheria toxin-like 10
      • ARTD10
      • PARP10/ARTD10
      Background InformationPoly [ADP-ribose] polymerase 10 (EC 2.4.2.30; UniProt Q53GL7; also known as ADP-ribosyltransferase diphtheria toxin-like 10, ARTD10, PARP-10) is encoded by the PARP10 gene (Gene ID 84875) in human. The ARTD/PARP family adenosine diphosphate (ADP)-ribosyltransferases share a conserved catalytic domain. They can be divided into three classes depending on their catalytic activity. The first class members can generate and modify their substrates with polymers of ADP-ribose (PAR), the second class consists of mono-ADP-ribosyltransferases, while the third class members possess no catalytic activity. ARTD10 (PARP10), originally identified as a MYC-interacting protein is the first recognized member of the second class of ARTD enzymes that target substrates for mono-ADP-ribose (mADPr) modification. ARTD10 is the only ARTD family member that contains two ubiquitin-interaction motifs (UIMs), which mediate interaction with polyubiquitin (pUb) chains with Lys63 (K63) linkage. ARTD10 is reported to function as a negative regulator against NF-κB-dependent gene activation by targeting NEMO for mono-ADP-ribosylation and by preventing NEMO ubiquitination with K63-pUb chains.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-PARP10/ARTD10 Antibody, clone 5H11 is validated for use in Western Blotting, Immunocytochemistry for the detection of PARP10/ARTD10.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected exogenously expressed ARTD10 in transfected Hela cells (Verheugd, P., et al. (2013). Nat. Commun. 4:1683).
      Western Blotting Analysis: An 1:500 dilution from a representative lot detected IFN-alpha-stimulated ARTD10 upregulation and siRNA-mediated ARTD10 downregulation in U2OS cells (Courtesy of Dr. Bernhard Lüscher, RWTH Aachen University, Germany).
      Western Blotting Analysis: A representative lot detected doxycycline-induced expression of both wild-type ARTD10 and the catalytically inactive G888W mutant in respective HeLa Flp–In T–REx clones (Herzog, N., et al. (2013). FASEB J. 280:1330–1343).
      Western Blotting Analysis: A representative lot detected increased ARTD10 N-terminal fragment (a.a. 1-406) in THP-1 cells upon apoptosis induction by etoposide or staurosporine treatment (Herzog, N., et al. (2013). FASEB J. 280:1330–1343).
      Western Blotting Analysis: A representative lot detected increased ARTD10 N-terminal fragment (a.a. 1-406) in U2OS cells and HeLa Flp–In T–REx clones upon apoptosis induction by UV or doxorubicin treatment (Herzog, N., et al. (2013). FASEB J. 280:1330–1343).
      Western Blotting Analysis: A representative lot detected exogenously expressed ARTD10 in transfected Hela and U2OS cells (Verheugd, P., et al. (2013). Nat. Commun. 4:1683).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the Glycine-rich region of human PARP10/ARTD10.
      EpitopeGlycine-rich region
      Clone5H11
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PARP10
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~135 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U20S cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected PARP10/ARTD10 in 10 µg of U20S cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-PARP10/ARTD10, clone 5H11 -Q2623740 Q2623740

      References

      Reference overviewPub Med ID
      Caspase-dependent cleavage of the mono-ADP-ribosyltransferase ARTD10 interferes with its pro-apoptotic function.
      Herzog, N; Hartkamp, JD; Verheugd, P; Treude, F; Forst, AH; Feijs, KL; Lippok, BE; Kremmer, E; Kleine, H; Lüscher, B
      The FEBS journal  280  1330-43  2013

      Show Abstract
      23305266 23305266
      Regulation of NF-?B signalling by the mono-ADP-ribosyltransferase ARTD10.
      Verheugd, P; Forst, AH; Milke, L; Herzog, N; Feijs, KL; Kremmer, E; Kleine, H; Lüscher, B
      Nature communications  4  1683  2013

      Show Abstract
      23575687 23575687