MABN1097 | Anti-PDE1B, clone 10B10B12 Antibody

MABN1097
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, FC, ELISARbAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABN1097
      DescriptionAnti-PDE1B, clone 10B10B12 Antibody
      Alternate Names
      • Calcium/calmodulin-dependent 3
      • 5'-cyclic nucleotide phosphodiesterase 1B
      • Cam-PDE 1B
      • 63 kDa Cam-PDE
      • PDE1B
      Background InformationPlasmid k1 killer toxin alpha-subunit gene DNA, 5' end (PDE1B) belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. PDE1B has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes.
      References
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Applications
      ApplicationAnti-PDE1B, clone 10B10B12 Antibody is an antibody against PDE1B for use in western blotting, flow cytometry & ELISA.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • ELISA
      Application NotesFlow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected PDE1B in A549 cells.

      ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
      Biological Information
      ImmunogenPurified recombinant fragment of human PDE1B expressed in E. Coli
      Clone10B10B12
      HostRabbit
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • PDE1B
      • PDE1B1
      • PDES1B
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~62 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549, SK-MES-1, PC-12, NIH/3T3 and 3T3-L1 cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PDE1B in A549, SK-MES-1, PC-12, NIH/3T3 and 3T3-L1 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies