AB10424 | Anti-Phospho-Bad (Ser99) Antibody

AB10424
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB10424
      DescriptionAnti-Phospho-Bad (Ser99) Antibody
      Alternate Names
      • BCL-X/BCL-2 binding protein
      • BCL2-antagonist of cell death protein
      • BCL2-associated agonist of cell death
      • BCL2-binding component 6
      • BCL2-binding protein
      • Bcl-2-binding component 6
      • Bcl-2-like 8 protein
      • Bcl-XL/Bcl-2-associated death promoter
      Background InformationBad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.
      References
      Product Information
      FormatPurified
      Control
      • Western Blot : HL60 lysates.
      PresentationPurified in PBS with 0.09% NaN3.
      Applications
      ApplicationThis Anti-Phospho-Bad (Ser99) Antibody is validated for use in WB for the detection of Phospho-Bad (Ser99).
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenThis antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S99 of human Bad.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThe antibody reacts with the 18 kDa Phospho-Bad (Ser99) protein.
      Species Reactivity
      • Human
      Species Reactivity NoteReacts with human. Other species have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq]
      Gene Symbol
      • BBC2
      • BBC6
      • BCL2L8
      Modifications
      • Phosphorylation
      Purification MethodProtein G followed by IAP
      UniProt Number
      UniProt SummaryFUNCTION: Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 By similarity. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
      SUBUNIT STRUCTURE: Forms heterodimers with the anti-apoptotic proteins, Bcl-X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 By similarity. The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins By similarity.
      SUBCELLULAR LOCATION: Mitochondrion outer membrane. Cytoplasm. Note= Upon phosphorylation, locates to the cytoplasm.
      TISSUE SPECIFICTY: Expressed in a wide variety of tissues.
      DOMAIN: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
      PTM: Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation By similarity.
      SEQUENCE SIMILARITIES: Belongs to the Bcl-2 family.
      SEQUENCE CAUTION: The sequence AAB36516.1 differs from that shown. Reason: Frameshift at positions 64 and 91.
      Molecular Weight18 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis: 1:250 dilution of this antibody detected Phospho-Bad-S99 on HL60 lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C for 6 months from date of receipt. For long term storage, store at -20°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies