04-577 | Anti-RasGAP Antibody, rabbit monoclonal, clone EP536Y

04-577
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RICC, IH(P), WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-577
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-RasGAP Antibody, rabbit monoclonal, clone EP536Y
      Background InformationRasGAP, a regulator of Ras and Rho, stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. RasGAP is a Caspase 3 substrate (1) and acts as both pro- and anti-apoptotic protein depending on the extent of its cleavage by caspases (2). At low levels of caspase activity, RasGAP is cleaved at position 455, generating an N-terminal fragment (fragment N) and a C-terminal fragment (fragment C). Fragment C alone can induce apoptosis, but this response is completely inhibited by fragment N (3). Fragment N appears to be a general blocker of apoptosis downstream of caspase activation because it inhibits caspase 9-induced cell death. At higher caspase activity, fragment N is further processed and Cleavage of fragment N abrogates its protective functions, and hence the second cleavage of RasGAP promotes apoptosis (4).
      References
      Product Information
      FormatPurified
      PresentationBuffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect RasGAP using this Anti-RasGAP Antibody, Rabbit, clone EP536Y validated for use in IC, IH(P) & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWB: 1:2000
      IHC: 1:50 (Paraffin-embedded human ovary carcinoma)
      ICC: 1:250 - 500 (Hela cells)
      Biological Information
      ImmunogenA synthetic peptide corresponding to residues near the SH3 domain of human RasGAP.
      CloneEP536Y
      HostRabbit
      SpecificityRecognizes Human, Rat RasGap.
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat (positive control: Rat brain lysate)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.
      Gene Symbol
      • RASA1
      • CMAVM
      • CM-AVM
      • RASA
      • PKWS
      • GAP
      • p120GAP
      • RASGAP
      • RasGAP
      • DKFZp434N071
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P20936 # Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.
      SIZE: 1047 amino acids; 116403 Da
      SUBUNIT: Interacts with SQTSM1.
      SUBCELLULAR LOCATION: Cytoplasm.
      PTM: The N-terminus is blocked.
      DISEASE: SwissProt: P20936 # Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas. & Defects in RASA1 are the cause of capillary malformation- arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome. & Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb.
      SIMILARITY: SwissProt: P20936 ## Contains 1 C2 domain. & Contains 1 PH domain. & Contains 1 Ras-GAP domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20 °C. Stable for 12 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RasGAP, Rabbit Monoclonal, clone EP536Y - 2051385 2051385
      Anti-RasGAP, Rabbit Monoclonal, clone EP536Y - DAM1554354 DAM1554354

      References

      Reference overviewPub Med ID
      Impaired Akt activity down-modulation, caspase-3 activation, and apoptosis in cells expressing a caspase-resistant mutant of RasGAP at position 157.
      Yang, JY; Walicki, J; Michod, D; Dubuis, G; Widmann, C
      Molecular biology of the cell  16  3511-20  2005

      Show Abstract
      15901831 15901831
      Surviving the kiss of death.
      Yang, Jiang-Yan, et al.
      Biochem. Pharmacol., 68: 1027-31 (2004)  2004

      Show Abstract
      15313397 15313397
      Partial cleavage of RasGAP by caspases is required for cell survival in mild stress conditions.
      Yang, JY; Michod, D; Walicki, J; Murphy, BM; Kasibhatla, S; Martin, SJ; Widmann, C
      Molecular and cellular biology  24  10425-36  2004

      Show Abstract
      15542850 15542850
      Antiapoptotic signaling generated by caspase-induced cleavage of RasGAP
      Yang, J Y and Widmann, C
      Mol Cell Biol, 21:5346-58 (2001)  2001

      11463818 11463818

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies