MABS760 | Anti-SPAK, clone EPR6394, Rabbit Monoclonal Antibody

MABS760
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS760
      DescriptionAnti-SPAK, clone EPR6394, Rabbit Monoclonal Antibody
      Alternate Names
      • STE20/SPS1-related proline-alanine-rich protein kinase
      • Ste-20-related kinase
      • STK39
      • SPAK
      Background InformationSerine/threonine-protein kinase 39 (STK39), also known as STE20/SPS-1 related proline alanine-rich kinase (SPAK), is a member of the SPS1 subfamily of STE20 kinases. STK39 is also very highly conserved between species and has been shown to activate the p38 pathways. STK39 also has a caspase cleavage site and these two functions may mean that STK39 plays a role in stress-activated signaling. STK39 expression is up-regulated by androgens in LNCaP human prostate cancer cells and regulated and phosphorylated by WNK1, which controls the stress signaling pathway. It is activated by WNK4 and then has the ability to stimulate the Na-K-2Cl cotransporter, the inactive version does not possess this ability. Recent research has also made a connection between STK39 and regulation of blood pressure.
      References
      Product Information
      FormatUnpurified
      PresentationRabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.
      Applications
      ApplicationAnti-SPAK, clone EPR6394, Rabbit Monoclonal is an antibody against SPAK for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50-100 dilution from a representative lot detected SPAK in human colon tissue.

      Produced in collaboration with Epitomics™
      Biological Information
      ImmunogenSynthetic peptide corresponding to human SPAK.
      CloneEPR6394
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityEquilibrium disassociation constant (KD) is 3.10 x 10 -11 M
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • STK39
      • SPAK
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~65 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SH-SY5Y, HEK293, Jurkat, BxPC3, and HepG2 cell lysates.

      Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected SPAK in SH-SY5Y, HEK293, Jurkat, BxPC3, and HepG2 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies