ABS1641 | Anti-SPTLC2 Antibody

ABS1641
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS1641
      DescriptionAnti-SPTLC2 Antibody
      Alternate Names
      • Serine palmitoyltransferase 2
      • hLCB2a
      • LCB 2
      • LCB2a
      • Long chain base biosynthesis protein 2
      • Long chain base biosynthesis protein 2a
      • Serine-palmitoyl-CoA transferase 2
      • SPT 2
      Background InformationSerine palmitoyltransferase 2 (EC 2.3.1.50; UniProt O15270; also known as hLCB2a, LCB 2, LCB2a, Long chain base biosynthesis protein 2, Long chain base biosynthesis protein 2a, Serine-palmitoyl-CoA transferase 2, SPT 2) is encoded by the SPTLC2 (also known as HSAN1C, KIAA0526, NSAN1C, LCB2) gene (Gene ID 9517) in human. Serine palmitoyltransferase (SPT) is a pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyses the first step of de novo sphingolipids (SLs) biosynthesis, namely the condensation of L-serine with palmitoyl coenzyme-A to generate 3-ketodehydrosphinganine (KDS) that forms the sphingoid base backbone of all SLs. The core human SPT homoenzyme is a membrane-bound heterodimer composed of an hLCB1 (SPTLC1) subunit together with either an hLCB2a (SPTLC2) or an hLCB2b (SPTLC3) subunit. The hLCB1/hLCB2 heterodimer contains a single active site composed of residues from both subunits. hLCB2a and hLCB2b share 68% sequence identity, with hLCB2b being the isotype that is predominantly expressed in the placenta and prefers shorter chain acyl-CoAs (C12 and C14) to generate short-chain SLs. In addition, two human small subunits (ssSPTa and ssSPTb) have been identified and found to maximize the catalytic activity and to confer the acyl-CoA substrate chain-length specificity of the hLCB1/hLCB2 core enzyme. Mutations in hLCB1 and hLCB2a are found in patients with hereditary sensory and autonomic neuropathy type I (HSAN1), an inherited disorder that affects sensory and autonomic neurons due to neurotoxic deoxysphingolipids formation as a result of substrate promiscuity of the mutated SPT enzyme.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody serum with 0.05% sodium azide.
      Applications
      ApplicationAnti-SPTLC2 Antibody is an antibody against SPTLC2 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: Representative lots detected SPTLC2 in human placenta tissue homogenates and in various lysates from human cell lines, including embryonic kidney HEK293, hepatocellular carcinoma HepG2, choriocarcinoma JAr and JEG-3 (Hornemann, T., et al. (2007). Biochem. J. 405(1):157-164; Hornemann, T., et al. (2006). J. Biol. Chem. 281(49):37275-37281).
      Biological Information
      ImmunogenA mixture of two linear peptides corresponding to sequences from the N-terminal region and the C-terminal end of human SPTLC2.
      EpitopeN-terminal region and C-terminal end.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityTarget-specificity of this antiserum was verified by negative blotting using pre-immune serum and by target band blocking using immunogen peptides (Hornemann, T., et al. (2006). J. Biol. Chem. 281(49):37275-37281).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Chicken, Bovine, Xenopus based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SPTLC2
      • HSAN1C
      • KIAA0526
      • NSAN1C
      • LCB2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~63 kDa observed. 62.92 kDa calculated. Target band size is also reported with a smaller apparent molecular weight of ~58kDa. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected SPTLC2 in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications