MABC528 | Anti-SSTR5/SS5R (P335L mutation) Antibody, clone D2F2

MABC528
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC528
      DescriptionAnti-SSTR5/SS5R (P335L mutation) Antibody, clone D2F2
      Alternate Names
      • Somatostatin receptor type 5
      • SS-5-R
      • SS5-R
      • SS5R
      Background InformationSomatostatin receptor subtype 5 (SSTR5) mediates the inhibitory effect of somatostatin on insulin expression/secretion and cell proliferation. A number of single nucleotide polymorphisms (SNPs) of SSTR5 have been identified, including P335L, a nonsynonymous SNP located in the protein C-terminal region and encrypted by the codon CCG (proline) or the codon CTG.

      SSTR5 SNP P335L widely exists in the human population, in patients with pancreatic cancer, and is race-dependent. SSTR5 SNP P335 overexpression resulted in cellular proliferation and PDX-1 overexpression in human pancreatic cancer cells. Its overexpression also blocked the inhibitory effect of SSTR5-specific analogs on human pancreatic cancer cell proliferation and on glucose-stimulated insulin secretion from mouse insulinoma cells. SSTR5 P335L is a hypofunctional protein with a potentially harmful effect on function, as well as potential latent effect, and therefore it could affect the clinical response to somatostatin analog therapy for patients with pancreatic cancer.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-SSTR5/SS5R (P335L mutation) Antibody, clone D2F2 is validated for use in western blotting, ICC & IHC for the detection of SSTR5/SS5R (P335L mutation).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected SSTR5/SS5R (P335L mutation) in normal human pancreas and human pancreatic cancer tissues.

      Western Blotting Analysis: A representative lot from an independent laboratory detected SSTR5/SS5R (P335L mutation) in HA-SSTR5 (P335L mutation) transfected HEK293 cell lysate (Zhou, G., et al. (2011). World J Surg. 35(8):1715-1724.).

      Immunocytochemistry Analysis: A representative lot from an independent laboratory detected SSTR5/SS5R (P335L mutation) in Panc-1 cells expressing SSTR5 (P335L mutation), and not in Panc-1 cells or CAPAN-1 cells expressing wild type SSTR5 (Zhou, G., et al. (2011). Surgery. 150(6):1136-1142.).

      Western Blotting Analysis: A representative lot from an independent laboratory detected SSTR5/SS5R (P335L mutation) in CAPAN-1 and Panc-1 cell lysates expressing SSTR5/SS5R (P335L mutation) (Zhou, G., et al. (2011). Surgery. 150(6):1136-1142.).
      Biological Information
      ImmunogenLinear peptide corresponding to human SSTR5/SS5R (P335L mutation).
      CloneD2F2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SSTR5
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~39 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Panc-1 cell lysate and Mia Paca-2 cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected SSTR5/SS5R (P335L mutation) in 10 µg of Panc-1 cell lysate and not in Mia Paca-2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-SSTR5/SS5R (P335L mutation), clone D2F2 - Q2384855 Q2384855

      References

      Reference overviewPub Med ID
      The hypofunctional effect of P335L single nucleotide polymorphism on SSTR5 function.
      Zhou, Guisheng, et al.
      World J Surg, 35: 1715-24 (2011)  2011

      Show Abstract
      21249361 21249361
      SSTR5 P335L monoclonal antibody differentiates pancreatic neuroendocrine neuroplasms with different SSTR5 genotypes.
      Zhou, Guisheng, et al.
      Surgery, 150: 1136-42 (2011)  2011

      Show Abstract
      22136833 22136833

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies