AB1594P | Anti-Serotonin Transporter Antibody

AB1594P
50 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RELISA, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1594P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Serotonin Transporter Antibody
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Liquid in 100 mM Tris, pH 7.5 with 0.2% BSA and 0.05% sodium azide.
      Applications
      ApplicationAnti-Serotonin Transporter Antibody is an antibody against Serotonin Transporter for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern blot: 1-10 μg/mL


      Cell pellets are resuspended in 100 μL lysis buffer (25 mM Tris, pH 7.6, 150 mM NaCl, 1 μg/mL aprotonin, 10 μg/mL leupeptin, 1 mM phenylmethylsulfonylfluoride, 1 mM sodium orthovanadate, 1 mM EDTA, and 1% Triton X-100). Samples were incubated for 1 hour at 4°C and were pelleted at high speed for 10 minutes at 4°C, and 100 μg protein per sample was subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis.





      ELISA: 1:100,000 primary EIA using immunogen only (50-100 ng SERT peptide (Catalog Number AG297)/well). Not tested on purified protein at the stated dilutions.


      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenA 15 amino acid sequence between transmembrane domain 7 and 8 of the rat serotonin transporter. This region is presumably from the extracellular domain.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificitySerotonin Transporter (SERT). An antibody raised against a similar peptide has been shown to react with a SERT in baculovirus cells expressing recombinant rat and human SERT. Antibody activity may be reduced considerably when detecting endogenous SERT from rat or human cells. SERT has been observed to migrate as 94 kDa (rat platelet), 80 kDa (pulmonary membranes) and 76 kDa (rat brain). This could be due to differences in postranslational membrane modifications (e.g. glycosylation).
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, and depression-susceptibility in people experiencing emotional trauma.
      Gene Symbol
      • SLC6A4
      • 5-HTT
      • 5HTT
      • HTT
      • OCD1
      • hSERT
      • SERT
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P31645 # Terminates the action of serotonin by its high affinity sodium-dependent reuptake into presynaptic terminals.
      SIZE: 630 amino acids; 70325 Da
      SUBUNIT: Interacts with TGFB1I1.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      SIMILARITY: SwissProt: P31645 ## Belongs to the sodium:neurotransmitter symporter (SNF) family.
      MISCELLANEOUS: This protein is the target of psychomotor stimulants such as amphetamines or cocaine.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-SEROTONIN TRANSPORTER AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2430342 2430342
      RABBIT ANTI-SEROTONIN TRANSPORTER - 2219278 2219278
      RABBIT ANTI-SEROTONIN TRANSPORTER AFFINITY PURIFIED POLYCLONAL ANTI - 2361795 2361795
      RABBIT ANTI-SEROTONIN TRANSPORTER AFFINITY PURIFIED POLYCLONAL ANTIBODY 2879729
      RABBIT ANTI-SEROTONIN TRANSPORTER AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2065980 2065980
      RABBIT ANTI-SEROTONIN TRANSPORTER AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2891413 2891413
      RABBIT ANTI-SEROTONIN TRANSPORTER, AFFINITY PURIFIED, POLYCLONAL ANTIBODY 2839605

      References

      Reference overviewApplicationSpeciesPub Med ID
      Effect of the 5-HT4 receptor and serotonin transporter on visceral hypersensitivity in rats.
      Yan, C; Xin-Guang, L; Hua-Hong, W; Jun-Xia, L; Yi-Xuan, L
      Brazilian journal of medical and biological research = Revista brasileira de pesquisas me?dicas e biolo?gicas / Sociedade Brasileira de Biofi?sica ... [et al.]  45  948-54  2012

      Show Abstract
      22832600 22832600
      Fate map of serotonin transporter-expressing cells in developing mouse thyroid.
      Spina A, Rea S, De Pasquale V, Mastellone V, Avallone L, Pavone LM.
      Anatomical record (Hoboken, N.J. : 2007)  294  384-90  2011

      Show Abstract
      21308996 21308996
      Regulation of serotonin transport in human platelets by tyrosine kinase Syk.
      Martina Pavanetto,Alessandro Zarpellon,Christian Borgo,Arianna Donella-Deana,Renzo Deana
      Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology  27  2011

      Show Abstract
      21325831 21325831
      Expression of the serotonin transporter SERT in the genital tract of cattle.
      Luigi M Pavone, Simona Tafuri, Roberta Lo Muto, Anna Spina, Alla E Lychkova, Vincenzo Mastellone, Alfredo Vittoria, Luigi Avallone, Norma Staiano, Luigi M Pavone, Simona Tafuri, Roberta Lo Muto, Anna Spina, Alla E Lychkova, Vincenzo Mastellone, Alfredo Vittoria, Luigi Avallone, Norma Staiano, Luigi M Pavone, Simona Tafuri, Roberta Lo Muto, Anna Spina, Alla E Lychkova, Vincenzo Mastellone, Alfredo Vittoria, Luigi Avallone, Norma Staiano
      Regulatory peptides  154  1-4  2009

      Show Abstract
      19323988 19323988
      Serotonin transporter gene deficiency is associated with sudden death of newborn mice through activation of TGF-beta1 signalling.
      Pavone LM, Spina A, Rea S, Santoro D, Mastellone V, Lombardi P, Avallone L
      Journal of molecular and cellular cardiology  47  691-697  2009

      Show Abstract
      19646988 19646988
      The cellular distribution of serotonin transporter is impeded on serotonin-altered vimentin network.
      Ahmed, BA; Bukhari, IA; Jeffus, BC; Harney, JT; Thyparambil, S; Ziu, E; Fraer, M; Rusch, NJ; Zimniak, P; Lupashin, V; Tang, D; Kilic, F
      PloS one  4  e4730  2009

      Show Abstract
      19270731 19270731
      Treadmill exercise enhances passive avoidance learning in rats: the role of down-regulated serotonin system in the limbic system.
      Hsiun-Ing Chen,Li-Chun Lin,Lung Yu,Yu-Fan Liu,Yu-Min Kuo,A-Min Huang,Jih-Ing Chuang,Fong-Sen Wu,Pao-Chi Liao,Chauying J Jen
      Neurobiology of learning and memory  89  2008

      Show Abstract
      17892954 17892954
      Psychostimulants differentially regulate serotonin transporter expression in thalamocortical neurons.
      Whitworth, Terri L, et al.
      J. Neurosci., 22: RC192 (2002)  2002

      Show Abstract
      Immunohistochemistry (Tissue)Rat11756522 11756522

      Data Sheet

      Title
      Anti-Serotonin Transporter - Data Sheet

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      AB10514P Anti-Serotonin Transporter Antibody Show Pricing & Availability
      AB1772 Anti-Serotonin Transporter Antibody Show Pricing & Availability
      AB9322 Anti-Serotonin Transporter Antibody Show Pricing & Availability
      AB9726 Anti-Serotonin Transporter Antibody Show Pricing & Availability
      MAB5618 Anti-Serotonin Transporter Antibody Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies