ABS527 | Anti-Sphk2, C-term Antibody

ABS527
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, IP, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS527
      DescriptionAnti-Sphk2, C-term Antibody
      Alternate Names
      • Sphingosine kinase 2
      • SK 2
      • SPK 2
      Background InformationSphingosine kinase 2 (Sphk2), along with its sister enzyme Sphk1, is an important kinase needed to form the important intra and extracellular signaling lipid mediator and neuroprotective factor SPP/S1P (sphingosine 1-phosphate). Sphk2 is important in blood vessel development, brain development, as well as an anti-apoptotic factor, and it mediates many proliferation mediated responses such as those surrounding insulin and its mitogenic ability. Sphk2 is localized to the lysosome membrane primarily but can also be found in the cytoplasm. Interestingly, recent research indicates that ApoE regulates the secretion Sphingosine 1-phosphate in the brain, and SPP/S1P in turn positively regulates glutamate secretion and synaptic strength in hippocampal neurons, thus Sphk1/2 plays an important role in synaptic plasticity and neuronal activity connectivity in the brain as well.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Sphk2, C-term Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of Sphk2, C-term.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Sphk2 in 10 µg of mouse lung tissue lysate.
      Western Blotting Analysis: A representative lot detected Sphk2 in mouse BMMC tissue lysate (Olivera, A., et al. (2006). JBC. 281(5):2515-2525).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated Sphk2 in mouse BMMC tissue lysate (Olivera, A., et al. (2006). JBC. 281(5):2515-2525).
      Immunocytochemistry Analysis: A representative lot detected Sphk2 in Huvec cells (Venkataraman, K., et al. (2006) Biochem. J. 397:461-471).
      Biological Information
      ImmunogenRecombinant protein corresponding to the C-terminus of mouse Sphk2, C-term.
      EpitopeC-terminus
      HostRabbit
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Sphk2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~65 kDa observed, uncharacterized bands may be observed at higher molecular weights
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in RAW264.7 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Sphk2, C-term in 10 µg of RAW264.7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Sphk2, C-term - Q2459306 Q2459306

      References

      Reference overviewPub Med ID
      IgE-dependent activation of sphingosine kinases 1 and 2 and secretion of sphingosine 1-phosphate requires Fyn kinase and contributes to mast cell responses.
      Olivera, Ana, et al.
      J. Biol. Chem., 281: 2515-25 (2006)  2006

      Show Abstract
      16316995 16316995
      Extracellular export of sphingosine kinase-1a contributes to the vascular S1P gradient.
      Venkataraman, Krishnan, et al.
      Biochem. J., 397: 461-71 (2006)  2006

      Show Abstract
      16623665 16623665