MABC584 | Anti-Sulf-2 Antibody, clone 2B4

MABC584
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC584
      DescriptionAnti-Sulf-2 Antibody, clone 2B4
      Alternate Names
      • Extracellular sulfatase Sulf-2
      • hSulf-2
      Background InformationSulf-2 is also important in neurological situations including myelination and glial cell differentiation and GDNF activity. Sulf-2 activity is also found in the kidney and important in glomerular basement membrane development and glomerular function. Finally, Sulf-2 is involved in heparan sulfate metabolism. Sulf-2 removes sulfate moieties on heparan sulfate proteoglycans which in turn modulate the heparin binding growth factor signaling activity of the cell. Sulf-2 also impacts extracellular matrix interactions and it is important during development and growth, as well as Wnt signaling and VEGF production by endothelial cells during vascularization. In disease, Sulf-2 expression in cancer is upregulated and inhibition of Sulf-2 limits cancer progression and showed increased apoptosis within tumors, thus Sulf-2 activation is being examined as a potential anti-cancer therapy.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Sulf-2 Antibody, clone 2B4 is validated for use in Western Blotting and Immunohistochemistry and ELISA for the detection of Sulf-2.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • ELISA
      Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot of this antibody detected Sulf-2 in transfected HEK293 cell lysates (Data courtesy of Dr. Mark Singer).

      Western Blot Analysis: A representative lot of this antibody detected Sulf-2 in transfected HEK293 cell lysates, but not in non-transfected HEK293 cell lysates (Lemjabbar-Alaoui, H., et al., (2010) Oncogene 29:635–646).

      Immunohistochemistry Analysis: A representative lot of this antibody detected Sulf-2 in Human lung and squamouse cell carcinoma tissue (Lemjabbar-Alaoui, H., et al., (2010) Oncogene 29:635–646).

      ELISA: A representative lot of this antibody detected Sulf-2, but not Sulf-1 by ELISA (Lemjabbar-Alaoui, H., et al., (2010) Oncogene 29:635–646).
      Biological Information
      ImmunogenRecombinant protein corresponding to Human Sulf-2.
      Clone2B4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SULF2
      • KIAA1247
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~125 & 50 kDa observed.
      Sulf-2 is synthesized as a ~125 kDa pre-protein and is subsequently cleaved into ~75 kDa and ~50 kDa fragments, which are held together by disulfide bonds in the mature protein.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected Sulf-2 in 10 µg of A549 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Sulf-2, clone 2B4 Q2471540
      Anti-Sulf-2, clone 2B4 -2770275 2770275

      References

      Reference overviewPub Med ID
      Sulf-2, a heparan sulfate endosulfatase, promotes human lung carcinogenesis.
      Lemjabbar-Alaoui, H; van Zante, A; Singer, MS; Xue, Q; Wang, YQ; Tsay, D; He, B; Jablons, DM; Rosen, SD
      Oncogene  29  635-46  2010

      Show Abstract
      19855436 19855436

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies