04-1133 | Anti-TIMP-1 Antibody, clone EP1549RY, rabbit monoclonal

04-1133
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1133
      DescriptionAnti-TIMP-1 Antibody, clone EP1549RY, rabbit monoclonal
      Alternate Names
      • Collagenase inhibitor
      • Erythroid-potentiating activity
      • Fibroblast collagenase inhibitor
      • TIMP metallopeptidase inhibitor 1
      • Tissue inhibitor of metalloproteinases
      Background InformationTissue Inhibitors of Metalloproteinases (TIMPs) inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-1 and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kDa type IV collagenase (also known as MMP-2), and latent and active forms 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-1 levels in quiescent cells and tissues are low (pg/ml), and stimulation with the phorbol ester TPA or protein concentration is often needed to visualize protein bands. In addition, cell types differ greatly in the amount of TIMP-1 produced.
      References
      Product Information
      FormatUnpurified
      Control
      • Recombinant protein
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-TIMP-1 Antibody, clone EP1549RY, Rabbit is an antibody against TIMP-1 for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues in the C-terminal region of human TIMP-1.
      EpitopeC-terminus
      CloneEP1549RY
      HostRabbit
      SpecificityThis antibody recognizes TIMP-1.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CLGI
      • EPA
      • EPO
      • FLJ90373
      • HCI
      • OTTHUMP00000023214
      • TIMP
      • TIMP-1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.

      SUBCELLULAR LOCATION: Secreted.

      PTM: The activity of TIMP1 is dependent on the presence of disulfide bonds.

      SEQUENCE SIMILARITIES: Belongs to the protease inhibitor I35 (TIMP) family. [View classification]

      Contains 1 NTR domain.

      Molecular Weight~ 28-30 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot.

      Western Blot Analysis: 1:100-500 dilution of this antibody was used to detect TIMP-1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.

      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies