ABE617 | Anti-TOP1 Antibody

ABE617
150 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE617
      DescriptionAnti-TOP1 Antibody
      Alternate Names
      • DNA topoisomerase 1
      • DNA topoisomerase I
      • TOP1
      Background InformationThe protein named DNA topoisomerase 1 and encoded by the human gene named TOP1 is the enzyme that plays the essential role in unwinding DNA necessary for DNA replication and transcription. TOP1 does so by inducing single-strand breaks via transesterification at the target site in duplex DNA. TOP1 requires ATP for breakage of the ssDNA and is specifically inhibited by camptothecin, a plant alkaloid with anti-tumor activity. Expressed in all cells but highly expressed in rapid dividing or highly expressing cells such as endothelial cells and liver cells. In disease, auto-antibodies against TOP1 are indicative of scleroderma autoimmune disease. Besides DNA TOP1 also interacts with a variety of proteins necessary to perform its primary function including BTBD1, P53, Nucleolin, BTBD2, ASF/SF2, and UBE2I. TOP1 is often up-regulated in cancers. We offer a rabbit polyclonal Anti-TOP1 Antibody that has been validated in western blot on L02 cell extracts.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide and 50% glycerol.
      Applications
      ApplicationDetect DNA Topoisomerase using this rabbit polyclonal antibody, Anti-TOP1 Antibody validated for use in western blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human TOP1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • TOP1
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~70 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in L02 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected TOP1 in L02 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size150 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies