MABT497 | Anti-TUBB2A, clone 6A10B2 Antibody

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ELISA, FCMPurifiedMonoclonal Antibody
      Catalogue NumberMABT497
      DescriptionAnti-TUBB2A, clone 6A10B2 Antibody
      Alternate Names
      • Tubulin beta-2A chain
      • Tubulin beta class IIa
      Background InformationTubulin beta-2A chain, also known as Tubulin beta class Iia, and encoded by the gene name TUBB2A or TUBB2 is a regulatory gene of mitosis and is a member of the Tubulin family. Tubulin is the major component of microtubules and binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB2A is highly expressed in brain, where it represents 30% of all beta-tubulins. TUBB2A has been shown to interact with ZNRF1 and is part of a complex composed of at least ASLC2, MATR3, HCFC1, and WDR5. The TUBB2A complex may have a histone H3-specific methyltransferase activity.
      Product Information
      PresentationPurified mouse monoclonal in PBS containing up to 0.1% sodium azide and 0.5% protein stabilizer.
      ApplicationAnti-TUBB2A, clone 6A10B2 Antibody is an antibody against TUBB2A for use in western blotting, ELISA & flow cytometry.
      Key Applications
      • Western Blotting
      • ELISA
      • Flow Cytometry
      Application NotesELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

      Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected TUBB2A in HeLa cells.
      Biological Information
      ImmunogenPurified recombinant fragment of human TUBB2A expressed in E. Coli
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • TUBB2A
      • TUBB2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~50 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa, A549, HEK293, Jurkat and PC-12 cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected TUBB2A in HeLa, A549, HEK293, Jurkat and PC-12 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information