MABS1245 | Anti-Thrombomodulin Antibody, clone 273-34A

MABS1245
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIHC, IP, IH(P), ELISA, EMRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1245
      DescriptionAnti-Thrombomodulin Antibody, clone 273-34A
      Alternate Names
      • TM
      • Thrombomodulin
      • Fetomodulin
      • CD141
      Background InformationThrombomodulin (UniProt P15306; also known as CD141, Fetomodulin, SnoRNA MBII-339, TM) is encoded by the Thbd gene (Gene ID 21824) in murine species. Thrombomodulin (TM) is an endothelial cell surface protein that function to suppress the propagation of thrombin (activated blood-coagulation factor II) generation by acting as a thrombin receptor. Formation of the TM-thrombin complex abolishes thrombin's procoagulant activity by altering its substrate specificity, leading to proteolytic cleavage of protein C into the natural anticoagulant termed "activated protein C". Together with protein S, activated protein C proteolytically cleaves activated coagulation factors Va and VIIIa, thereby preventing the generation of activated coagulation factors Xa and thrombin. Murine thrombomodulin is initially produced with an N-terminal propeptide (a.a. 1-16) sequence, the removal of which yields the mature protein with a large extracellular region (a.a. 17-517), a transmembrane segment (a.a. 518-541), and a short cytoplamic tail (a.a. 542-577).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Thrombomodulin Antibody, clone 273-34A is validated for use in Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin), ELISA, Electron Microscopy for the detection of Thrombomodulin.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      • ELISA
      • Electron Microscopy
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Thrombomodulin in human kidney tissue.
      Immunoprecipitation Analysis: Representative lots immunoprecipitated the 112 kDa thrombomodulin from normal mouse lung fibroblasts (NMLF), but not Line 1 mouse lung carcinoma cells (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701; Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124).
      Immunohistochemistry Analysis: A representative lot detected upregulated thrombomodulin immunoreactivity associated with the neoepidermis during wound healing using formalin-fixed, paraffin-embedded mouse skin sections (Peterson, J.J., et al. (1999). Am. J. Pathol. 155(5): 1569–1575).
      Immunohistochemistry Analysis: Representative lots detected epithelium thrombomodulin immunoreactivity in paraffin-embedded mouse lung sections (Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450; Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124).
      Immunohistochemistry Analysis: A representative lot detected thrombomodulin immunoreactivity associated with capillary endothelial cells by fluorescent immunohistochemistry using frozen mouse lung sections (Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124)
      ELISA Analysis: Representative lots were used as the capture antibody for the detection of thrombomodulin in mouse tissue samples by sandwich ELISA using clone 411-201B (Cat. No. MABS1273) as the detection antibody, followed by an HRP-conjugated secondary antibody, or by sandwich radio-immunoassay using 125I-labeled clone 411-201B as the detection antibody (Isermann, B., et al. (2001). Development. 128(6):827-838; Conway, E.M., et al. (1999). Blood. 93(10):3442-3450; Weiler-Guettler, H., et al. (1996). Circ. Res. 78(2):180-187; Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450).
      Electron Microscopy Analysis: Representative lots detected thrombomodulin immunoreactivity associated with capillary endothelial cells by EM using frozen mouse lung sections (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701; Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124).
      Western Blotting Analysis: A representative lot detected purified thrombomodulin (P112) from normal mouse lung fibroblasts (NMLF) (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).
      Biological Information
      ImmunogenNormal mouse lung microsomal fraction.
      EpitopeExtracellular domain
      Clone273-34A
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Thbd
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~ 62 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in mouse heart tissue.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Thrombomodulin in mouse heart tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Thrombomodulin, clone 273-34A -Q2623741 Q2623741

      References

      Reference overviewPub Med ID
      Tissue-restricted expression of thrombomodulin in the placenta rescues thrombomodulin-deficient mice from early lethality and reveals a secondary developmental block.
      Isermann, B; Hendrickson, SB; Hutley, K; Wing, M; Weiler, H
      Development (Cambridge, England)  128  827-38  2001

      Show Abstract
      11222138 11222138
      Structure-function analyses of thrombomodulin by gene-targeting in mice: the cytoplasmic domain is not required for normal fetal development.
      Conway, EM; Pollefeyt, S; Cornelissen, J; DeBaere, I; Steiner-Mosonyi, M; Weitz, JI; Weiler-Guettler, H; Carmeliet, P; Collen, D
      Blood  93  3442-50  1999

      Show Abstract
      10233896 10233896
      Externalization of tropomyosin isoform 5 in colon epithelial cells.
      Kesari, KV; Yoshizaki, N; Geng, X; Lin, JJ; Das, KM
      Clinical and experimental immunology  118  219-27  1999

      Show Abstract
      10540182 10540182
      Targeting of transgene expression to the vascular endothelium of mice by homologous recombination at the thrombomodulin locus.
      Weiler-Guettler, H; Aird, WC; Husain, M; Rayburn, H; Rosenberg, RD
      Circulation research  78  180-7  1996

      Show Abstract
      8575060 8575060
      Thrombomodulin is preferentially expressed in Balb/c lung microvessels.
      Ford, VA; Stringer, C; Kennel, SJ
      The Journal of biological chemistry  267  5446-50  1992

      Show Abstract
      1372003 1372003
      Quantitation of a murine lung endothelial cell protein, P112, with a double monoclonal antibody assay.
      Kennel, SJ; Lankford, T; Hughes, B; Hotchkiss, JA
      Laboratory investigation; a journal of technical methods and pathology  59  692-701  1988

      Show Abstract
      2460698 2460698
      Rat monoclonal antibodies to mouse lung components for analysis of fibrosis.
      Kennel, SJ; Hotchkiss, JA; Rorvik, MC; Allison, DP; Foote, LJ
      Experimental and molecular pathology  47  110-24  1987

      Show Abstract
      2440715 2440715

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies