MABT129 | Anti-VE-Cadherin Antibody (CD144), clone BV9

MABT129
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, EAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT129
      DescriptionAnti-VE-Cadherin Antibody (CD144), clone BV9
      Alternate Names
      • Cadherin-5
      • 7B4 antigen
      • Vascular endothelial cadherin
      • VE-cadherin
      • CD144
      Background InformationAlso known as CD144, human Vascular Endothelial (VE)-cadherin is a calcium-dependent adhesion molecule strictly located at cell to cell junctions. VE-cadherin is present in all types of endothelium (veins, arteries, capillary and large vessels). Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherins are reported to play an important role in angiogeneis and in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha catenin forming a link to the cytoskeleton.
      References
      Product Information
      FormatPurified
      Control
      • HUVEC cells
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing PBS without 0.05% sodium azide.
      Applications
      ApplicationThis VE-Cadherin antibody is validated for use in ICC & Enzyme Assay for the detection of the VE-Cadherin protein.
      Key Applications
      • Immunocytochemistry
      • Enzyme Assay
      Application NotesActivity Assay Analysis: A representative lot from an independent laboratory increased endothelial permeability, inhibited the organization of vasclular-like structures, and prevented the protective effect of VEGF against apoptosis in endothelial cells.
      Biological Information
      ImmunogenRecombinant protein corresponding to the extracellular domain of human VE-Cadherin.
      EpitopeExtracellular domain.
      CloneBV9
      HostMouse
      SpecificityThis antibody recognizes the extracellular domain of VE-Cadherin.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined.
      Gene Symbol
      • CDH5
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.

      SUBUNIT STRUCTURE: Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1.

      SUBCELLULAR LOCATION: Cell junction. Cell membrane; Single-pass type I membrane protein. Note: Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions.
      Molecular Weight88 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvalutated by Immunocytochemistry in HUVEC cells.

      Immunocytochemistry Analysis: 1 µg/mL of this antibody detected VE-Cadherin in HUVEC cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-VE-Cadherin (CD144), clone BV9 - 2142190 2142190
      Anti-VE-Cadherin (CD144), clone BV9 - 2080291 2080291
      Anti-VE-Cadherin (CD144), clone BV9 - 2266771 2266771
      Anti-VE-Cadherin (CD144), clone BV9 - 2366042 2366042
      Anti-VE-Cadherin (CD144), clone BV9 - NRG1929416 NRG1929416
      Anti-VE-Cadherin (CD144), clone BV9 -2567124 2567124
      Anti-VE-Cadherin (CD144), clone BV9 -2607662 2607662
      Anti-VE-Cadherin (CD144), clone BV9 -2620660 2620660
      Anti-VE-Cadherin (CD144), clone BV9 -2628844 2628844
      Anti-VE-Cadherin (CD144), clone BV9 -2676720 2676720

      References

      Reference overviewPub Med ID
      Monoclonal antibodies directed to different regions of vascular endothelial cadherin extracellular domain affect adhesion and clustering of the protein and modulate endothelial permeability.
      Corada, M; Liao, F; Lindgren, M; Lampugnani, MG; Breviario, F; Frank, R; Muller, WA; Hicklin, DJ; Bohlen, P; Dejana, E
      Blood  97  1679-84  2001

      Show Abstract
      11238107 11238107
      Inhibition of cultured cell growth by vascular endothelial cadherin (cadherin-5/VE-cadherin).
      Caveda, L; Martin-Padura, I; Navarro, P; Breviario, F; Corada, M; Gulino, D; Lampugnani, MG; Dejana, E
      The Journal of clinical investigation  98  886-93  1996

      Show Abstract
      8770858 8770858
      Functional properties of human vascular endothelial cadherin (7B4/cadherin-5), an endothelium-specific cadherin.
      Breviario, F; Caveda, L; Corada, M; Martin-Padura, I; Navarro, P; Golay, J; Introna, M; Gulino, D; Lampugnani, MG; Dejana, E
      Arteriosclerosis, thrombosis, and vascular biology  15  1229-39  1995

      Show Abstract
      7627717 7627717
      A novel endothelial-specific membrane protein is a marker of cell-cell contacts.
      Lampugnani, MG; Resnati, M; Raiteri, M; Pigott, R; Pisacane, A; Houen, G; Ruco, LP; Dejana, E
      The Journal of cell biology  118  1511-22  1992

      Show Abstract
      1522121 1522121

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies