ABS99 | Anti-Wrch-1 Antibody

ABS99
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS99
      DescriptionAnti-Wrch-1 Antibody
      Alternate Names
      • Rho-related GTP-binding protein RhoU
      • CDC42-like GTPase 1
      • GTP-binding protein-like 1
      • Rho GTPase-like protein ARHU
      • Ryu GTPase
      • Wnt-1 responsive Cdc42 homolog 1
      • WRCH-1
      • Wrch-1
      Background InformationWrch-1 (Wnt-1 responsive Cdc42 homolog 1; Rho-related GTP-binding protein RhoU; GTP-binding protein-like 1; or CDC42-like GTPase 1) is a ubiquitous protein belonging to the Rho family of small GTPases. Wrch-1 is structurally and functionally similar to Cdc42 small GTPase but is distinctive in containing N-terminal SH3-binding motifs and exhibiting high intrinsic guanine nucleotide exchange activity. Wrch-1 is regulated by the Wnt-1 signaling pathway. It is a key determinant of cell morphology as it is involved in cell adhesion and cytoskeleton organization.
      References
      Product Information
      FormatAffinity Purified
      Control
      • C2C12 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Wrch-1 Antibody is validated for use in Western Blotting, IHC(P) for the detection of Wrch-1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Wrch-1 in mouse colon and mouse pancreas tissues.
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to human Wrch-1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation. A non-coding transcript variant of this gene results from naturally occurring read-through transcription between this locus and the neighboring DUSP5P (dual specificity phosphatase 5 pseudogene) locus.[provided by RefSeq, Mar 2011].
      Gene Symbol
      • RHOU
      • ARHU
      • CDC42L1
      • G28K
      • WRCH1
      • SB128
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.

      COFACTOR: Magnesium.

      SUBUNIT STRUCTURE: Interacts with PAK3. Interacts with ARHGAP30 in a GTP-independent manner. In its GTP-loaded conformation, interacts with ARHGAP31.

      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Cell junction › focal adhesion. Cell projection › podosome. Note: Localizes to podosomes in SRC-transformed cells.

      TISSUE SPECIFICITY: Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach, small intestine, brain, skeletal muscle and placenta.

      SEQUENCE SIMILARITIES: Belongs to the small GTPase superfamily. Rho family.
      Molecular Weight~29 kDa observed. UniProt describes 2 isoforms at 28 kDa and 22 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in C2C12 cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Wrch-1 on 10 µg of C2C12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications