MABE193 | Anti-YBOX1/YB-1 Antibody, clone EP2706Y, rabbit monoclonal

MABE193
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FC, IPRbDiluted Culture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMABE193
      DescriptionAnti-YBOX1/YB-1 Antibody, clone EP2706Y, rabbit monoclonal
      Alternate Names
      • Y box binding protein 1
      • nuclease sensitive element binding protein 1
      • Enhancer factor I subunit A
      • Y-box transcription factor
      • CCAAT-binding transcription factor I subunit A
      • DNA-binding protein B
      • Y-box-binding protein 1
      • nuclease-sensitive element-binding protein 1
      • major histocompatibility complex, class II, Y box-binding protein I
      Background InformationHuman DNA-binding proteins, dbpA and dbpB (YBOX-1), are members of a protein family containing a coldshock domain, and are regarded as transcriptional regulators (1). The expression of HLA class II genes is regulated by a series of cis-acting elements and trans-acting factors. Several cis-acting elements have been identified and have been termed the Z box, X box, Y box, octamer, and "TATA" box. The Y box contains an inverted CCAAT box. An inverse correlation of YB-1 and HLA-DR beta chain mRNA levels suggests that YB-1 is a negative regulatory factor (2). Overexpression of YB-1 resulted in increased levels of PTP1B. Furthermore, depletion of YB-1 protein, by expression of a specific antisense construct, led to an approximately 70% decrease in expression of PTP1B, but no change in the level of its closest relative, TC-PTP. Data reveal an important role for YB-1 as a regulator of PTP1B expression, and further highlight PTP1B as a critical regulator of insulin- and cytokine-mediated signal transduction (3).
      References
      Product Information
      FormatDiluted Culture Supernatant
      Control
      • HeLa, 293T, Jurkat, and MCF7 cell lysates
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationAnti-YBOX1/YB-1 Antibody, clone EP2706Y is a Rabbit Monoclonal Antibody for detection of YBOX1/YB-1 also known as Y box binding protein 1 & has been validated in WB, FC & IP.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 1:60 dilution from a representative lot was used in IP.
      Flow Cytometry Analysis: 1:50 dilution from a representative lot detected YBOX1 in permeabilized HeLa cells.
      Biological Information
      ImmunogenSynthetic peptide corresponding to human YBOX1 near the N-terminus.
      EpitopeNear N-terminus
      CloneEP2706Y
      HostRabbit
      SpecificityThis antibody recognizes YBOX1 near the N-terminus.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • DBPB
      • YB1
      • YB-1
      • NSEP1
      • NSEP-1
      • CSDB
      • BP-8
      • CSDA2
      • MDR-NF1
      • EFI-A
      • CBF-A
      • OTTHUMP00000038221
      • MGC117250
      • MGC104858
      • MGC110976
      • OTTHUMP00000008732
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors By similarity. Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.
      The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.
      SUBUNIT STRUCTURE: Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1 By similarity. Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Interacts with IGF2BP1 and RBBP6. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, MBNL1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP. Interacts (via C-terminus) with APEX1 (via N-terminus); the interaction is increased with APEX1 acetylated at 'Lys-6' and 'Lys-7'.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.
      PTM: Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcativational ability.
      In the absence of phosphorylation the protein is retained in the cytoplasm.
      Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus By similarity.
      SEQUENCE SIMILARITIES: Contains 1 CSD (cold-shock) domain.
      SEQUENCE CAUTION: The sequence AAA35750.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
      The sequence AAA59949.1 differs from that shown. Reason: Frameshift at several positions.
      The sequence AAA61308.1 differs from that shown. Reason: Frameshift at position 314.
      Molecular Weight~49 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa, 293T, Jurkat, and MCF7 cell lysates.
      Western Blot Analysis: 1:20,000 dilution of this antibody detected YBOX1 in HeLa, 293T, Jurkat, and MCF7 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below
      -20°C may cause glycerol containing Solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies