MABT888 | Anti-ZO-2 Antibody, clone Vli13

MABT888
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIH(P)RbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMABT888
      DescriptionAnti-ZO-2 Antibody, clone Vli13
      Alternate Names
      • Tight junction protein ZO-2
      • Tight junction protein 2
      • Zona occludens protein 2
      • Zonula occludens protein 2
      • ZO-2
      Background InformationTight junction protein ZO-2 (UniProt Q9Z0U1; also known as Tight junction protein 2, Zona occludens protein 2, Zonula occludens protein 2) is encoded by the Tjp2 (also known as Zo2) gene (Gene ID 21873) in murine species. The tight junction (TJ) constitutes the barrier between the apical and the basolateral domains of the plasma membrane. The assembly and permeability of this barrier are dependent on the zonula occludens (ZO) membrane-associated guanylate kinase (MAGUK) proteins ZO-1, -2, and -3 found at the submembranous domain of TJs in epithelia and endothelia. The 160 kDa ZO-2 contains three PDZ domains (PDZ1/a.a. 10-97, PDZ2/a.a. 287-365, PDZ3/a.a. 489-570) at its N-terminal end, followed by an SH3 domain (a.a. 584-649), a GUK/GK homology domain (a.a. 660-858), and a short PDZ-binding motif (TEL; a.a. 1165-1167) at the C-terminus. In addition to associating with various transmembrane junction proteins via its C-terminal PDZ-binding motif, ZO-2 is also involved in modulating gene expression and cell proliferation by interacting with a number of proteins in the nucleus. Evidence suggests that ZO-2 is also involved in stress response and cytoprotective mechanisms.
      References
      Product Information
      FormatCulture Supernatant
      PresentationRabbit monoclonal antibody in tissue culture supernatant with 0.05% sodium azide.
      Applications
      ApplicationDetect ZO-2 using this Anti-ZO-2 Antibody, clone Vli13 validated for use in Immunohistochemistry (Paraffin).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ZO-2 in human heart and spleen tissue sections.
      Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected ZO-2 in rat heart myocardium and brain tissue sections (Courtesy of Dr. Volkhard Lindner, Tufts University School of Medicine, ME, USA).
      Biological Information
      ImmunogenLinear peptide corresponding to a C-terminal sequence of mouse ZO-2.
      EpitopeC-terminal region.
      CloneVli13
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityExpected to react with human ZO-2 spliced isoforms A1, A2, C1, C2, 6, and 7, but not A3, reported by UniProt (Q9UDY2).
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tjp2
      • Zo2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight134.0 kDa (isoform A1), 117.8 kDa (isoform A2), 131.4 kDa (isoform C1), 115.2 kDa (isoform C2), 130.4 kDa (isoform 6), and 137.3 kDa (isoform 7) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in mouse heart tissue.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected ZO-2 in mouse heart tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications