07-354 | Anti-acetyl-Histone H3 (Lys18) Antibody

07-354
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Vrt, Yeast (S. cerevisiae)WB, DB, ChIP, ChIP-seqRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-354
      Replaces04-1107
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H3 (Lys18) Antibody
      Alternate Names
      • H3K18Ac
      • Histone H3 (acetyl K18)
      Background InformationHistone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

      Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      References
      Product Information
      FormatSerum
      Control
      • Acid extracted proteins from HeLa cells treated with sodium butyrate
      Presentation100 μLof rabbit serum containing 0.05% sodium azide and 30% glycerol.
      Applications
      ApplicationUse Anti-acetyl-Histone H3 (Lys18) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB to detect acetyl-Histone H3 (Lys18) also known as H3K18Ac, Histone H3 (acetyl K18).
      Key Applications
      • Western Blotting
      • Dot Blot
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      Application NotesChromatin Immunoprecipitation:
      Representative lot data.
      Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either Normal Rabbit Serum , or 2 µL of Anti-Acetyl-Histone H3 (Lys18)and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of Acetyl-Histone H3 (Lys18) associated DNA fragments was verified by qPCR using Control Primers specific for the human GAPDH promoter region as a positive locus, and β-globin primers as a negative locus. Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
      Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

      ChIP-Sequencing:
      Representative lot data. Chromatin immunoprecipitation was performed using the Magna ChIP™ HiSens kit (cat# 17-10460), 2 μg of anti-acetyl-Histone H3 (Lys18) antibody (cat# 07-354), 20 µL Protein A/G beads , and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq™ instrument. An excess of ten million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files.

      Western Blot Analysis:
      Representative lot data.
      Recombinant histone H3 (lane 1, Catalog # 14-494) and acid extracts from sodium butyrate treated (lane 2) and untreated (lane 3) HeLa cells (Catalog # 17-305) were probed with anti acetyl- Histone H3 (Lys18) (1:10,000 dilution).
      Arrow indicates acetyl histone H3 (Lys18) (17 kDa).

      Dot Blot:
      Representative lot data.
      40 ng and 4ng amounts of histone peptides with various modifications (see table 1) were transferred to PVDF membrane and probed with Anti-Acetyl-Histone H3 (Lys18) antibody (1:5000 dilution). Proteins were visualized using a goat anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system. Image from a 60 second exposure is shown.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (GKAPRAcKQLASK-C) corresponding to amino acids 13-23 of yeast Histone H3 acetylated on lysine 18with a C-terminal cysteine added for conjugation purposes
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Histone H3 acetylated on lysine 18.
      Species Reactivity
      • Human
      • Vertebrates
      • Yeast (S. cerevisiae)
      Species Reactivity NoteYeast, Human, Most common vertebrates
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • MGC126886
      • H3t
      • MGC126888
      • H3T
      • H3/g
      • H3.4
      • H3/t
      Modifications
      • Acetylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on acid extracts from sodium butyrate treated HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 (Lys18) (rabbit antiserum) - 2152224 2152224
      Anti-acetyl-Histone H3 (Lys18) (rabbit antiserum) - 2117167 2117167
      Anti-acetyl-Histone H3 (Lys18) Polyclonal Antibody - 2450192 2450192
      Anti-acetyl-Histone H3 (Lys18) (rabbit antiserum) - 2037642 2037642
      Anti-acetyl-Histone H3 (Lys18) - 22401 22401
      Anti-acetyl-Histone H3 (Lys18) - 2510379 2510379
      Anti-acetyl-Histone H3 (Lys18) - 27132 27132
      Anti-acetyl-Histone H3 (Lys18) - DAM1661073 DAM1661073
      Anti-acetyl-Histone H3 (Lys18) - DAM1780381 DAM1780381
      Anti-acetyl-Histone H3 (Lys18) - DAM1817674 DAM1817674

      References

      Reference overviewApplicationSpeciesPub Med ID
      Nucleosome competition reveals processive acetylation by the SAGA HAT module.
      Ringel, AE; Cieniewicz, AM; Taverna, SD; Wolberger, C
      Proceedings of the National Academy of Sciences of the United States of America  112  E5461-70  2015

      Show Abstract
      26401015 26401015
      The adenovirus E1A oncoprotein N-terminal transcriptional repression domain enhances p300 autoacetylation and inhibits histone H3 Lys18 acetylation.
      Zhao, LJ; Loewenstein, PM; Green, M
      Genes & cancer  6  30-7  2015

      Show Abstract
      Western Blotting25821559 25821559
      Establishment of Trophectoderm Cell Lines from Buffalo (Bubalus bubalis) Embryos of Different Sources and Examination of In Vitro Developmental Competence, Quality, Epigenetic Status and Gene Expression in Cloned Embryos Derived from Them.
      Mohapatra, SK; Sandhu, A; Singh, KP; Singla, SK; Chauhan, MS; Manik, R; Palta, P
      PloS one  10  e0129235  2015

      Show Abstract
      26053554 26053554
      Histone H3.3 and its proteolytically processed form drive a cellular senescence programme.
      Duarte, LF; Young, AR; Wang, Z; Wu, HA; Panda, T; Kou, Y; Kapoor, A; Hasson, D; Mills, NR; Ma'ayan, A; Narita, M; Bernstein, E
      Nature communications  5  5210  2014

      Show Abstract
      25394905 25394905
      Active, phosphorylated fingolimod inhibits histone deacetylases and facilitates fear extinction memory.
      Hait, NC; Wise, LE; Allegood, JC; O'Brien, M; Avni, D; Reeves, TM; Knapp, PE; Lu, J; Luo, C; Miles, MF; Milstien, S; Lichtman, AH; Spiegel, S
      Nature neuroscience  17  971-80  2014

      Show Abstract
      Western BlottingMouse24859201 24859201
      The bromodomain of Gcn5 regulates site specificity of lysine acetylation on histone H3.
      Cieniewicz, AM; Moreland, L; Ringel, AE; Mackintosh, SG; Raman, A; Gilbert, TM; Wolberger, C; Tackett, AJ; Taverna, SD
      Molecular & cellular proteomics : MCP  13  2896-910  2014

      Show Abstract
      25106422 25106422
      Human leukocyte antigen-G is frequently expressed in glioblastoma and may be induced in vitro by combined 5-aza-2'-deoxycytidine and interferon-? treatments: results from a multicentric study.
      Wastowski, IJ; Simões, RT; Yaghi, L; Donadi, EA; Pancoto, JT; Poras, I; Lechapt-Zalcman, E; Bernaudin, M; Valable, S; Carlotti, CG; Flajollet, S; Jensen, SS; Ferrone, S; Carosella, ED; Kristensen, BW; Moreau, P
      The American journal of pathology  182  540-52  2013

      Show Abstract
      23219427 23219427
      A Common histone modification code on C4 genes in maize and its conservation in Sorghum and Setaria italica.
      Heimann, L; Horst, I; Perduns, R; Dreesen, B; Offermann, S; Peterhansel, C
      Plant physiology  162  456-69  2013

      Show Abstract
      23564230 23564230
      Cigarette smoke component acrolein modulates chromatin assembly by inhibiting histone acetylation.
      Chen, D; Fang, L; Li, H; Tang, MS; Jin, C
      The Journal of biological chemistry  288  21678-87  2013

      Show Abstract
      23770671 23770671
      Antisilencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis.
      Li, X; Qian, W; Zhao, Y; Wang, C; Shen, J; Zhu, JK; Gong, Z
      Proceedings of the National Academy of Sciences of the United States of America  109  11425-30  2012

      Show Abstract
      Western Blotting22733760 22733760

      Technical Info

      Title
      Anti-acetyl-Histone H3 (Lys18) Antibody ChIP-Seq Analysis
      Anti-acetyl-Histone H3 (Lys18) ChIP-Seq Analysis

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