07-593 | Anti-acetyl-Histone H3 (Lys9/18) Antibody

07-593
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, Yeast (S. cerevisiae)WB, Mplex, ChIPRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-593
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H3 (Lys9/18) Antibody
      Alternate Names
      • H3K9/18Ac
      • Histone H3 (acetyl K9/18)
      Background InformationHistone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

      Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      References
      Product Information
      FormatSerum
      Control
      • Acid extracts from sodium butyrate treated HeLa cells
      PresentationProtein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationAnti-acetyl-Histone H3 (Lys9/18) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H3 (Lys9/18) also known as H3K9/18Ac, Histone H3 (acetyl K9/18) & has been validated in ChIP, WB, Mplex.
      Key Applications
      • Western Blotting
      • Multiplexing
      • Chromatin Immunoprecipitation (ChIP)
      Biological Information
      Immunogenpeptide (ARAcKSTGGKAPRAcKQL) which corresponds to acetyl-lysine at residue 9 and 18 of human Histone H3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Histone H3 acetylated on lysines 9 and 18.
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Yeast (S. cerevisiae)
      Species Reactivity NoteBroad species cross-reactivity expected
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Gene Symbol
      • H3F3A
      • MGC87783
      • H3.3A
      • MGC87782
      • H3F3
      • H3.3B
      • H3F3B
      Modifications
      • Acetylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on acid extracts from sodium butyrate treated Hela cells (Catalog #17-305)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 (Lys9/18) (rabbit antiserum) 2929174
      Anti-acetyl-Histone H3 (Lys9/18) (rabbit antiserum) - 1966940 1966940
      Anti-acetyl-Histone H3 (Lys9/18) (rabbit antiserum) - 2032854 2032854
      Anti-acetyl-Histone H3 (Lys9/18) - 0605029104 0605029104
      Anti-acetyl-Histone H3 (Lys9/18) - 0701049661 0701049661
      Anti-acetyl-Histone H3 (Lys9/18) - 1350558 1350558
      Anti-acetyl-Histone H3 (Lys9/18) - 1975343 1975343
      Anti-acetyl-Histone H3 (Lys9/18) - 2517883 2517883
      Anti-acetyl-Histone H3 (Lys9/18) - 26975 26975
      Anti-acetyl-Histone H3 (Lys9/18) - DAM1594198 DAM1594198

      References

      Reference overviewApplicationSpeciesPub Med ID
      Pharmacological targeting of the β-amyloid precursor protein intracellular domain.
      Branca, C; Sarnico, I; Ruotolo, R; Lanzillotta, A; Viscomi, AR; Benarese, M; Porrini, V; Lorenzini, L; Calzà, L; Imbimbo, BP; Ottonello, S; Pizzi, M
      Scientific reports  4  4618  2014

      Show Abstract
      24714650 24714650
      Melatonin induces histone hyperacetylation in the rat brain.
      Niles, Lennard P, et al.
      Neurosci. Lett., (2013)  2013

      Show Abstract
      23416321 23416321
      Epigenetics of hypoxic pulmonary arterial hypertension following intrauterine growth retardation rat: epigenetics in PAH following IUGR.
      Xu, XF; Lv, Y; Gu, WZ; Tang, LL; Wei, JK; Zhang, LY; Du, LZ
      Respiratory research  14  20  2013

      Show Abstract
      23406533 23406533
      1B/(-)IRE DMT1 expression during brain ischemia contributes to cell death mediated by NF-κB/RelA acetylation at Lys310.
      Rosaria Ingrassia,Annamaria Lanzillotta,Ilenia Sarnico,Marina Benarese,Francesco Blasi,Laura Borgese,Fabjola Bilo,Laura Depero,Alberto Chiarugi,Pier Franco Spano,Marina Pizzi
      PloS one  7  2012

      Show Abstract
      22666436 22666436
      Plant flavone apigenin inhibits HDAC and remodels chromatin to induce growth arrest and apoptosis in human prostate cancer cells: In vitro and in vivo study.
      Mitali Pandey,Parminder Kaur,Sanjeev Shukla,Ata Abbas,Pingfu Fu,Sanjay Gupta
      Molecular carcinogenesis  51  2012

      Show Abstract
      22006862 22006862
      All-Trans Retinoic Acid Induces Chromatin Remodeling at the Promoter of the Mouse Liver, Bone, and Kidney Alkaline Phosphatase Gene in C3H10T 1/2 Cells.
      Yang Wan,Songhai Yang,Fenyong Sun,Jiayi Wang,Qiongyu Chen,An Hong
      Biochemical genetics  50  2012

      Show Abstract
      22270475 22270475
      Serum starvation induces DRAM expression in liver cancer cells via histone modifications within its promoter locus.
      Ni, P; Xu, H; Chen, C; Wang, J; Liu, X; Hu, Y; Fan, Q; Hou, Z; Lu, Y
      PloS one  7  e50502  2012

      Show Abstract
      23251372 23251372
      Green tea polyphenols induce p53-dependent and p53-independent apoptosis in prostate cancer cells through two distinct mechanisms.
      Gupta, K; Thakur, VS; Bhaskaran, N; Nawab, A; Babcook, MA; Jackson, MW; Gupta, S
      PloS one  7  e52572  2012

      Show Abstract
      23285096 23285096
      Nsi1 plays a significant role in the silencing of ribosomal DNA in Saccharomyces cerevisiae.
      Ha, CW; Sung, MK; Huh, WK
      Nucleic acids research  40  4892-903  2012

      Show Abstract
      22362748 22362748
      Green tea polyphenols causes cell cycle arrest and apoptosis in prostate cancer cells by suppressing class I histone deacetylases.
      Thakur, VS; Gupta, K; Gupta, S
      Carcinogenesis  33  377-84  2012

      Show Abstract
      Western Blotting22114073 22114073

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