ABE186 | Anti-acetyl-Histone H4 (Lys77) Antibody

ABE186
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, DBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE186
      DescriptionAnti-acetyl-Histone H4 (Lys77) Antibody
      Alternate Names
      • Histone H4
      Background InformationHistones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli. Acetylation is a reversible PTM that involves the transfer of acetyl groups to N-terminal lysine (K) residues by the family of histone acetyltransferase enzymes, including GNAT, Gcn5, and p300/CBP. Histone acetylation reduces the electrostatic attractions between DNA and histones, which destabilizes chromatin structure and provide chromatin remodeling and transcriptional complexes with access to DNA, potentially resulting in increased gene transcription.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Untreated and sodium butyrate treated HeLa acid extract, and recombinant Histone H4 protein
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-acetyl-Histone H4 (Lys77) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-Histone H4 (Lys77) also known as H4K77Ac, Histone H4 (acetyl K77) & has been validated in WB, DB.
      Key Applications
      • Western Blotting
      • Dot Blot
      Application NotesDot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with Anti-acetyl-Histone H4 (Lys77) (1:320 dilution).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to yeast Histone H4 acetylated at Lys77.
      EpitopeAcetylated Lys77
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Histone H4 acetylated at Lys77.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human and Mouse. Predicted to react with Yeast based on immunogen design. Broad species reactivity is expected based on similar sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HHF1
      • HHF2
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~13 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and sodium butyrate treated HeLa acid extract, and recombinant Histone H4 protein.

      Western Blot Analysis: A 1:320 dilution of this antibody detected Histone H4 acetylated at Lys77 in 10 µg of untreated and sodium butyrate treated HeLa acid extract, and recombinant Histone H4 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies