OP97 | Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2)

OP97
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody

      Pricing & Availability

      Catalogue NumberAvailability Packaging Qty/Pack Price Quantity
      OP97-100UG
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      Limited AvailabilityLimited Availability
      In Stock 
      Discontinued
      Limited Quantities Available
      Availability to be confirmed
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~290 kDa mTOR protein in HEK293 and Jurkat cells.
          Catalogue NumberOP97
          Brand Family Calbiochem®
          Application Data
          Detection of mTOR by immunoblotting. Sample: Whole cell lysate (50 μg) from HEK293 cells. Primary antibody: Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2) (1.7 μg/ml). Detection: chemiluminescence.
          References
          ReferencesKimura N et al. 2003. Genes Cells 8, 65.
          Sekulic A et al. 2000. Cancer Res. 60, 3504.
          Hosoi, H. 1999. Cancer Res. 59, 886.
          Alarcon, C.M., et al. 1996. Genes Dev. 10, 279.
          Freeman, K., and Livi, G.P. 1996. Gene 172, 143.
          Hosoi, H., et al. 1996. AACR 87th Ann. Mtg. Abstract 3445.
          Cardenas, M.E., and Heitman, J. 1995. EMBO J. 14, 5892.
          Lorenz, M.C., and Heitman, J. 1995. J. Biol. Chem. 270, 27531.
          Sabatini, D.M., et al. 1995. J. Biol. Chem. 270, 20875.
          Zheng, X.F., et al. 1995. Cell 82, 121.
          Brown, E.J., et al. 1994. Nature 369, 756.
          Sabatini, D.M., et al. 1994. Cell 78, 35.
          Stan, R., et al. 1994. J. Biol. Chem. 269, 32027.
          Kunz, J., et al. 1993. Cell 73, 585.
          Heitman, J., et al. 1991. Science 253, 905.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
          Positive controlJurkat or HeLa cells
          Preservative≤ 0.1% sodium azide
          Applications
          Application ReferencesImmunoblotting and Immunoprecipitation Hosoi, H. 1998. Mol. Pharmacol. 54, 815.
          Key Applications Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesImmunoblotting (1.7 µg/ml, see application references)
          Immunoprecipitation (1-10 µg/ml, see application references)
          Application CommentsThe immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids 230-240 of human TOR
          ImmunogenHuman
          Clone22C2
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          OP97

          References

          Reference overview
          Kimura N et al. 2003. Genes Cells 8, 65.
          Sekulic A et al. 2000. Cancer Res. 60, 3504.
          Hosoi, H. 1999. Cancer Res. 59, 886.
          Alarcon, C.M., et al. 1996. Genes Dev. 10, 279.
          Freeman, K., and Livi, G.P. 1996. Gene 172, 143.
          Hosoi, H., et al. 1996. AACR 87th Ann. Mtg. Abstract 3445.
          Cardenas, M.E., and Heitman, J. 1995. EMBO J. 14, 5892.
          Lorenz, M.C., and Heitman, J. 1995. J. Biol. Chem. 270, 27531.
          Sabatini, D.M., et al. 1995. J. Biol. Chem. 270, 20875.
          Zheng, X.F., et al. 1995. Cell 82, 121.
          Brown, E.J., et al. 1994. Nature 369, 756.
          Sabatini, D.M., et al. 1994. Cell 78, 35.
          Stan, R., et al. 1994. J. Biol. Chem. 269, 32027.
          Kunz, J., et al. 1993. Cell 73, 585.
          Heitman, J., et al. 1991. Science 253, 905.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision03-October-2007 RFH
          ApplicationImmunoblotting (1.7 µg/ml, see application references)
          Immunoprecipitation (1-10 µg/ml, see application references)
          Application Data
          Detection of mTOR by immunoblotting. Sample: Whole cell lysate (50 μg) from HEK293 cells. Primary antibody: Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2) (1.7 μg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SpAG8 mouse myeloma cells. Recognizes the ~290 kDa mTOR/FRAP protein.
          BackgroundTOR1 is one of three genes (FPR1, TOR1, and TOR2) mutations of which confer rapamycin resistance in the yeast Saccharomyces cerevisiae. The proteins encoded by the TOR1 and TOR2 genes are large (>250 kDa) in size, have homology to the phosphatidylinositol and protein kinases, and TOR2 possesses PI 4-kinase activity. The TOR proteins and their mammalian homolog (FRAP, mTOR) form a complex with a 12 kDa cytosolic protein, FKBP-12 and rapamycin to effect arrest in the G1 phase of the cell cycle. The G1 block is mediated by the activation of ribosomal p70S6K and cyclin dependent kinases. The cell cycle arrest activity requires the presence of the intact kinase domain in the TOR protein and in particular a conserved Ser residue within the domain. The human hTOR (mammalian or mTOR) protein shows 39-43% homology with the S. cerevisiae proteins TOR1 and TOR2. Ser2448 has been identified as a PKB-mediated phosphorylation site. The phosphorylation of Ser2448 on mTOR is directly related to amino acid and nutrient status. More recently it has been suggested that this nutrient effect on mTOR might act through AMPK as activation of this kinase is associated with reduced signaling through mTOR and reduced phosphorylation of Ser2448. This suggests that phosphorylation of Ser2448 might act as a switch, controlling the activity and function of mTOR.
          HostMouse
          Immunogen speciesHuman
          Immunogena synthetic peptide corresponding to amino acids 230-240 of human TOR
          Clone22C2
          IsotypeIgG₁
          Specieshuman
          Positive controlJurkat or HeLa cells
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤ 0.1% sodium azide
          CommentsThe immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesKimura N et al. 2003. Genes Cells 8, 65.
          Sekulic A et al. 2000. Cancer Res. 60, 3504.
          Hosoi, H. 1999. Cancer Res. 59, 886.
          Alarcon, C.M., et al. 1996. Genes Dev. 10, 279.
          Freeman, K., and Livi, G.P. 1996. Gene 172, 143.
          Hosoi, H., et al. 1996. AACR 87th Ann. Mtg. Abstract 3445.
          Cardenas, M.E., and Heitman, J. 1995. EMBO J. 14, 5892.
          Lorenz, M.C., and Heitman, J. 1995. J. Biol. Chem. 270, 27531.
          Sabatini, D.M., et al. 1995. J. Biol. Chem. 270, 20875.
          Zheng, X.F., et al. 1995. Cell 82, 121.
          Brown, E.J., et al. 1994. Nature 369, 756.
          Sabatini, D.M., et al. 1994. Cell 78, 35.
          Stan, R., et al. 1994. J. Biol. Chem. 269, 32027.
          Kunz, J., et al. 1993. Cell 73, 585.
          Heitman, J., et al. 1991. Science 253, 905.
          Application referencesImmunoblotting and Immunoprecipitation Hosoi, H. 1998. Mol. Pharmacol. 54, 815.