MABS196 | Anti-mTOR/FRAP Antibody, clone 22C2

MABS196
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS196
      Replaces04-385
      DescriptionAnti-mTOR/FRAP Antibody, clone 22C2
      Alternate Names
      • Serine/threonine-protein kinase mTOR
      • FK506-binding protein 12-rapamycin complex-associated protein 1
      • FKBP12-rapamycin complex-associated protein
      • Mammalian target of rapamycin
      • mTOR
      • Mechanistic target of rapamycin
      • Rapamycin and FKBP12 target 1
      • Rapamycin target protein 1
      Background InformationThe mammalian target of rapamycin (mTOR), also known as FRAP, RAFT, or RAPT, is a ubiquitously expressed serine/threonine kinase that belongs to the phosphoinositide 3-kinase–related kinase (PIKK) family. mTOR functions as a downstream effector in the PI3K pathway and is activated by the Akt kinase. mTOR responds to signals from growth factors and promotes protein synthesis underlying cell proliferation, by activation of the S6 kinase and inhibition of the 4E-BP protein involved in protein translation. Several studies have suggested that the proliferative functions of mTOR may contribute to the development of cancer.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect the mTOR/FRAP protein using this Anti-mTOR Antibody, clone 22C2, validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blot Analysis: A representative lot was used by an independent laboratory in neuroblastoma cells. (Hosoi, H., et al. (1998). Molecular Pharmacology. 54:815–824.)

      Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in Rho30 cell extracts. (Hosoi, H., et al. (1998). Molecular Pharmacology. 54:815–824.)
      Biological Information
      ImmunogenLinear peptide corresponding to human mTOR/FRAP.
      Clone22C2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse and Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MTOR
      • FRAP
      • FRAP1
      • FRAP2
      • RAFT1
      • RAPT1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~290 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Jurkat cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR/FRAP on 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-mTOR/FRAP, clone 22C2 - 2391153 2391153
      Anti-mTOR/FRAP, clone 22C2 - Q2010834 Q2010834
      Anti-mTOR/FRAP, clone 22C2 -2571253 2571253
      Anti-mTOR/FRAP, clone 22C2 -2643610 2643610
      Anti-mTOR/FRAP, clone 22C2 -2659646 2659646
      Anti-mTOR/FRAP, clone 22C2 Monoclonal Antibody 2912000
      Anti-mTOR/FRAP, clone 22C2 Monoclonal Antibody 2903975

      References

      Reference overviewPub Med ID
      Studies on the mechanism of resistance to rapamycin in human cancer cells.
      Hosoi, H, et al.
      Mol. Pharmacol., 54: 815-24 (1998)  1998

      Show Abstract
      9804616 9804616

      Brochure

      Title
      New Products: Volume 3, 2012

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies