05-929 | Anti-p19ARF Antibody, clone 5-C3-1

05-929
1 mL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIP, WB, IHCRCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number05-929
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-p19ARF Antibody, clone 5-C3-1
      References
      Product Information
      FormatCulture Supernatant
      Applications
      ApplicationAnti-p19ARF Antibody, clone 5-C3-1 is a high quality Rat Monoclonal Antibody for the detection of p19ARF & has been validated in IP, WB & IHC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 54-75 (RRGPHRNPG-PGDDDGQRSRSSS) of mouse p19ARF. The epitope is mapped to amino acids 62-75. Clone 5-C3-1.
      Cloneclone 5-C3-1
      HostRat
      Specificityp19ARF
      IsotypeIgG2b
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
      Gene Symbol
      • CDKN2A
      • CDKN2
      • P16
      • CMM2
      • ARF
      • p19ARF
      • p14ARF
      • TP16
      • p16
      • MTS1
      • INK4a
      • MLM
      • p16INK4a
      • INK4
      • p16-INK4a
      • p14
      • p16-INK4
      • p16INK4A
      • p19
      • p16INK4
      • CDK4I
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q8N726 # Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2- induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development.| P42771 # Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.


      SIZE: 173 amino acids; 18006 Da

      SUBUNIT: Does not interact with cyclins, CDC2, CDK2, CDK4, CDK5 or CDK6. Binds to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI and UBE2I/UBC9. Interacts with TBRG1.

      SUBCELLULAR LOCATION: Nucleus, nucleolus (By similarity).





      DISEASE: SwissProt: P42771 # Defects in CDKN2A are involved in tumor formation in a wide range of tissues. & Defects in CDKN2A are the cause of cutaneous malignant melanoma 2 (CMM2) [MIM:155601]. Inheritance is autosomal dominant. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a preexisting benign nevus, which occurs most often in the skin but also may involve other sites. & Defects in CDKN2A are the cause of familial atypical multiple mole melanoma-pancreatic carcinoma syndrome (FAMMMPC) [MIM:606719]. & Defects in CDKN2A are a cause of Li-Fraumeni syndrome (LFS) [MIM:151623]. LFS is a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. & Defects in CDKN2A are the cause of melanoma-astrocytoma syndrome [MIM:155755]. The melanoma-astrocytoma syndrome is characterized by a dual predisposition to melanoma and neural system tumors, commonly astrocytoma.

      SIMILARITY: SwissProt: P42771 ## Belongs to the CDKN2 cyclin-dependent kinase inhibitor family. & Contains 4 ANK repeats.
      Molecular Weight19kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-p19ARF, clone 5-C3-1 (rat culture supernatant) 2916013
      Anti-p19ARF, clone 5-C3-1 (rat culture supernatant) 2872446
      Anti-p19ARF, clone 5-C3-1 - 30471 30471
      Anti-p19ARF, clone 5-C3-1 -2608395 2608395
      Anti-p19ARF, clone 5-C3-1 -2795665 2795665

      References

      Reference overviewApplicationPub Med ID
      C/EBPbeta represses p53 to promote cell survival downstream of DNA damage independent of oncogenic Ras and p19(Arf).
      Ewing, SJ; Zhu, S; Zhu, F; House, JS; Smart, RC
      Cell death and differentiation  15  1734-44  2008

      Show Abstract
      Immunohistochemistry18636078 18636078
      Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein
      Bertwistle, D., et al
      Hybrid Hybridomics, 23:293-300 (2004)  2004

      Immunohistochemistry (Tissue)15672607 15672607

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies