MABE900 | Anti-pan Ago, clone 13A2 Antibody

MABE900
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE900
      DescriptionAnti-pan Ago, clone 13A2 Antibody
      Alternate Names
      • Protein argonaute-1
      • Argonaute1
      • mAgo1
      • Argonaute RISC catalytic component 1
      • Eukaryotic translation initiation factor 2C 1
      • eIF-2C 1
      • eIF2C 1
      • Piwi/argonaute family protein meIF2C1
      Background InformationArgonaute (Ago) proteins are a family of proteins of ~95 kDa that bind directly to mature miRNAs. In mammals, there are four Ago proteins (Ago1-4). This family of proteins is a central component of most mammalian miRNPs that mediates important functions in small RNA-directed regulatory pathways. The Ago family consists of two subclases, Ago and Piwi containing two characteristic domains known as PAZ and PIWI. These two domains interact with different parts of the mature miRNA. The PAZ domain interacts with the 3’-end of the miRNA, and the PIWI domain interacts with the 5’ phosphate and 5’-proximal region of the miRNA to guide target mRNA recognition.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect pan Ago using this Anti-pan Ago antibody, clone 13A2 validated for use in western blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected pan-Ago in mouse kidney and mouse liver tissue.
      Biological Information
      Clone13A2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ago1
      • Eif2c1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~97 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected pan-Ago in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-pan Ago, clone 13A2 - Q2385784 Q2385784

      References

      Reference overviewPub Med ID
      T cell activation induces proteasomal degradation of Argonaute and rapid remodeling of the microRNA repertoire.
      Bronevetsky, Y; Villarino, AV; Eisley, CJ; Barbeau, R; Barczak, AJ; Heinz, GA; Kremmer, E; Heissmeyer, V; McManus, MT; Erle, DJ; Rao, A; Ansel, KM
      The Journal of experimental medicine  210  417-32  2013

      Show Abstract
      23382546 23382546