ABE566 | Anti-phospho Histone H3 (Tyr41) Antibody| ABE566

ABE566
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, DBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE566
      DescriptionAnti-phospho Histone H3 (Tyr41) Antibody| ABE566
      Alternate Names
      • Histone H3.1
      • Histone H3/a
      • Histone H3/b
      • Histone H3/c
      • Histone H3/d
      • Histone H3/f
      • Histone H3/h
      • Histone H3/i
      • Histone H3/j
      • Histone H3/k
      • Histone H3/l
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-phospho Histone H3 (Tyr41) Antibody is validated for use in western blotting, ICC & dot blot for the detection of phospho Histone H3 (Tyr41).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Dot Blot
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho Histone H3 (Tyr41) in HeLa and NIH/3T3 cells.

      DyLight® is a registered trademark of Thermo Fisher Scientific.

      Dot Blot Analysis: 0.5 µg/mL from a representative lot detected phospho Histone H3 (Tyr41) in an Absurance® Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance® Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3 phosphorylated at Tyr41.
      EpitopeNear N-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in colcemid treated HeLa acid extract.

      Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H3 (Tyr41) in 10 µg of colcemid treated HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications