05-1087 | Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2

05-1087
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Mk, B, Ca, PoWB, IP, ICC, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1087
      Replaces07-247
      DescriptionAnti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2
      Alternate Names
      • insulin receptor substrate 1
      Background InformationIRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2 is validated for use in WB, IP, IC, IF for the detection of phospho-IRS1 (Ser307 mouse/ Ser312 human).
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunofluorescence
      Application NotesImmunocytochemistry:
      IR/IRS transfected CHO cells were stained with a 1:500 dilution of the antibody. A seconday anti-mouse Cy3 conjugate was used for visualizaion.

      Immunoprecipition:
      This antibody was used at a 1:1,000 to immunoprecipitate IRS1 from 5L/mL IR/IRS1 transfected CHO cells (performed by an independent lab).
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids surrounding phosphorylated Ser307 of mouse IRS1.
      EpitopePhosphorylated Ser307
      Clone24.6.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes IRS1 phosphorylated at Ser307 (mouse) and Ser312 (human).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Monkey
      • Bovine
      • Canine
      • Pig
      Species Reactivity NoteHuman, Mouse, and Rat. Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIRS-1
      • IRS-1
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit By similarity.

      SUBUNIT STRUCTURE: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts By similarity. Interacts with SOCS7.

      PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor By similarity.

      Phosphorylation of Tyr-896 is required for GRB2-binding By similarity.

      Polymorphism The Arg-971 polymorphism impairs the ability of insulin to stimulate glucose transport, glucose transporter translocation, and glycogen synthesis by affecting the PI3K/AKT1/GSK3 signaling pathway. The polymorphism at Arg-971 may contribute to the in vivo insulin resistance observed in carriers of this variant. Arg-971 could contribute to the risk for atherosclerotic cardiovascular diseases associated with non-insulin-dependent diabetes mellitus (NIDDM) by producing a cluster of insulin resistance-related metabolic abnormalities. In insulin-stimulated human endothelial cells from carriers of the Arg-971 polymorphism, genetic impairment of the IRS1/PI3K/PDPK1/AKT1 insulin signaling cascade results in impaired insulin-stimulated nitric oxide (NO) release and suggested that this may be a mechanism through which the Arg-971 polymorphism contributes to the genetic predisposition to develop endothelial dysfunction and cardiovascular disease. The Arg-971 polymorphism not only reduces phosphorylation of the substrate but allows IRS1 to act as an inhibitor of PI3K, producing global insulin resistance.

      DISEASE: Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. Ref.18 Ref.19 Ref.21 Ref.24 Ref.25

      SIMILARITIY: Contains 1 IRS-type PTB domain.

      Contains 1 PH domain.

      Molecular Weight~185 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on IR/IRS1 transfected CHO +/- Insulin lysate.

      Western Blot Analysis:
      1:1,000 dilution of this antibody was used to detect IRS1 in IRS/IR transfected CHO -/+ Calyculin A/ Okadaic Acid-treated cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-IRS1 (Ser307 mouse -2638874 2638874
      Anti-phospho-IRS1 (Ser307 mouse/ -2551187 2551187
      Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human), clone 24.6.2 - 2140062 2140062
      Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human), clone 24.6.2 - 2450196 2450196
      Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human), clone 24.6.2 - 2090139 2090139
      Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human), clone 24.6.2 - 2197312 2197312
      Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human), clone 24.6.2 - 2279270 2279270
      Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human), clone 24.6.2 - 2536321 2536321
      Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human), clone 24.6.2 - JBC1787320 JBC1787320
      Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human), clone 24.6.2 - NG1605287 NG1605287

      References

      Reference overviewPub Med ID
      Insulin and metabolic stress stimulate multisite serine/threonine phosphorylation of insulin receptor substrate 1 and inhibit tyrosine phosphorylation.
      Hançer, NJ; Qiu, W; Cherella, C; Li, Y; Copps, KD; White, MF
      The Journal of biological chemistry  289  12467-84  2014

      Show Abstract
      24652289 24652289

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      Life Science Research > Antibodies and Assays > Primary Antibodies