MABS175 | Anti-phospho KSR1 (Ser404) Antibody, clone EP2358Y, rabbit monoclonal

MABS175
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbDiluted Culture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMABS175
      DescriptionAnti-phospho KSR1 (Ser404) Antibody, clone EP2358Y, rabbit monoclonal
      Alternate Names
      • kinase suppressor of ras 1
      • kinase suppressor of ras
      Background InformationKinase suppressor of Ras (KSR) is a conserved component of the Ras pathway that interacts directly with MEK and MAPK. It has been shown that KSR1 translocates from the cytoplasm to the cell surface in response to growth factor treatment and that this process is regulated by MARK3 (1). MARK3 phosphorylates serine 309 and serine 404 on KSR1. While, PPP2CA dephosphorylates serine 404 on KSR1. MARK3 seems to be a positive regulator of the beta-catenin pathway and an inhibitor of the JNK pathway. These findings show that MARK3, a regulator of polarity, is also a modulator of Wnt-beta-catenin signaling, indicating a link between two important developmental pathways (2).
      References
      Product Information
      FormatDiluted Culture Supernatant
      Control
      • Calyculin A untreated and treated HeLa cell lysates
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationAnti-phospho KSR1 (Ser404) Antibody, clone EP2358Y, Rabbit is an antibody against phospho KSR1 (Ser404) for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:100-1:250 dilution from a representative lot detected KSR1 in Sh-SY-5Y cells.
      Biological Information
      ImmunogenSynthetic phospho-peptide corresponding to human KSR1 phosphorylated Ser404.
      EpitopePhosphorylated Ser404
      CloneEP2358Y
      HostRabbit
      SpecificityThis antibody recognizes KSR1 phosphorylated at Ser404.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KSR
      • RSU2
      • OTTHUMP00000162931
      Modifications
      • Phosphorylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNTION: Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
      SUBUNIT STRUCTURE: Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner By similarity. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization.
      SUBCELLULAR LOCATION: Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity. Note: In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery By similarity.
      PTM: Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated By similarity.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
      Molecular Weight~110 observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Calyculin A untreated and treated HeLa cell lysates.
      Western Blot Analysis: 1:2,000 dilution of this antibody detected KSR1 in Calyculin A untreated and treated HeLa cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies