07-1531 | Anti-phospho-PFKFB2 (Ser466) Antibody

200 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, B, Xn, PmWB, PIARbAffinity PurifiedPolyclonal Antibody
      Catalogue Number07-1531
      DescriptionAnti-phospho-PFKFB2 (Ser466) Antibody
      Alternate Names
      • 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
      • 6PF-2-K/Fru-2,6-P2ase 2
      • PFK/FBPase 2
      • 6PF-2-K/Fru-2,6-P2ase heart-type isozyme
      • 6-phosphofructo-2-kinase
      • Fructose-2,6-bisphosphatase
      Background Information6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (PFKFB2) is an enzyme involved in both the synthesis and degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart (a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle) to regulate glycolysis and gluconeogensis.
      Product Information
      FormatAffinity Purified
      • Untreated and insulin-treated HEK293 cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-phospho-PFKFB2 (Ser466) Antibody detects level of phospho-PFKFB2 (Ser466) & has been published & validated for use in WB, PIA.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inihibition Analysis: A 1:500 dilution from a representative lot blocked PFKFB2 in PANC-1 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human PFKFB2 phosphorylated at (Ser466).
      EpitopePhosphorylated Ser466
      SpecificityThis antibody recognizes PFKFB2 phosphorylated at Ser466.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Bovine
      • Xenopus
      • Primate
      Species Reactivity NoteDemonstrated to react with Human.
      Predicted to react with Rat, Mouse, Bovine, Xenopus, and Primate based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
      Gene Symbol
      • PFKFB2
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Synthesis and degradation of fructose 2,6-bisphosphate.

      CATALYTIC ACTIVITY: Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.

      ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

      ENZYME REGULATION: Phosphorylation results in the activation of the kinase activity.

      SUBUNIT STRUCTURE: Homodimer (By similarity).


      SEQUENCE SIMILARITIES: In the C-terminal section; belongs to the phosphoglycerate mutase family.
      Molecular Weight~58 kDa observed.
      Uniprot describes two isoforms at 54 kDa (isoform 1) and 58 kDa (isoform 2).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Insulin untreated and treated Hek293 cell lysates.

      Western Blot Analysis: A 1:500 dilution of this antibody detected PFKFB2 in 10 µg of untreated and insulin-treated HEK293 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies