MABF118 | Anti-sTRAIL Antibody, clone III6F

MABF118
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IHC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF118
      DescriptionAnti-sTRAIL Antibody, clone III6F
      Alternate Names
      • Tumor necrosis factor ligand superfamily member 10
      • Apo-2 ligand
      • Apo-2L
      • TNF-related apoptosis-inducing ligand
      • Protein TRAIL
      • CD253
      Background InformationTNF-related apoptosis-inducing ligand (sTRAIL or Apo-2L) is the endogenous ligand for the ubiquitous pro-apoptotic TRAIL-R1 and TRAIL-R2 receptors. Upon ligand binding, TRAIL receptors form trimeric structures at the plasma membrane around a central zinc atom. The trimeric TRAIL receptors bind the FADD adaptor protein which in turn recruits caspase 8. TRAIL/APO-2L signals ultimately converge on caspase 3 and related proteins that promote cell death. Importantly, activated TRAIL receptors work cooperatively with death receptors 4 and 5 in transformed cells to induce caspase signaling, thereby preventing the proliferation of abnormal cells. TRAIL/ApO-2L has been implicated in pathophysiology of many disease processes, including cancer and autoimmune diseases.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cells
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-sTRAIL Antibody, clone III6F is validated for use in Flow Cytometry, IHC, Immunoprecipitation for the detection of sTRAIL.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A representative lot from an independent laboratory detected sTRAIL in frozen sections from human tonsils, lymph nodes and appendix (Liabakk, N. B., et al. (2002). J Immunol Methods. 259(1-2):119-128.).

      Flow Cytometry Analysis: A representative lot from an independent laboratory detected sTRAIL in REH cells (Liabakk, N. B., et al. (2002). J Immunol Methods. 259(1-2):119-128.).
      Biological Information
      ImmunogenHuman soluble TRAIL
      CloneIII6F
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • TNFSF10
      • APO2L
      • TRAIL
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

      COFACTOR: Binds 1 zinc ion per trimer.

      SUBUNIT STRUCTURE: Homotrimer.

      SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein.

      TISSUE SPECIFICITY: Widespread; most predominant in spleen, lung and prostate.

      SEQUENCE SIMILARITIES: Belongs to the tumor necrosis factor family.
      Molecular Weight33 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in HeLa cells.

      Flow Cytometry Analysis: 2.5 µg/mL of this antibody detected sTRAIL in 2X10E5 HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-sTRAIL, clone III6F - 2394057 2394057
      Anti-sTRAIL, clone III6F - Q1998583 Q1998583

      References

      Reference overviewPub Med ID
      Development, characterization and use of monoclonal antibodies against sTRAIL: measurement of sTRAIL by ELISA.
      Liabakk, Nina Beate, et al.
      J. Immunol. Methods, 259: 119-28 (2002)  2002

      Show Abstract
      11730847 11730847

      Brochure

      Title
      New Products: Volume 3, 2012

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies