KAA002 | Cellular Senescence Assay

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      Key Spec Table

      Detection Methods
      Catalogue NumberKAA002
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionCellular Senescence Assay
      OverviewCellular senescence is one of the most fundamental aspects of cell behavior, and is thought to play a critical role in regulating cellular lifespan both in vitro and in vivo [1-3]. Primary somatic cells grown in vitro do not proliferate indefinitely. Instead, after a period of rapid proliferation, cell division rate slows, and ultimately ceases altogether, with the cells becoming unresponsive to mitogenic stimuli. This process is termed cellular senescence, and senescent cells have a well-defined accompanying phenotype - increased cell size, distinctive flat morphology, accumulated lipofuscin granules, wide changes in gene expression, and activity of senescence-associated β-galactosidase (SA-β-gal) [2,3].

      It is generally believed that cellular senescence reflects some of the changes that occur during the aging of organisms, and senescent cells have been detected in vivo at sites of age-related pathology, such as benign hyperplastic prostate [4] and atherosclerotic lesions [5]. Recent studies have also provided convincing demonstrations of cellular senescence occurring in vivo in response to internally- and externally-induced stress signals [6,7]. In all of these studies, senescence was characterized by the appearance of senescence-associated β-galactosidase (SA-β-gal) activity, in common with the senescent phenotype in vitro.

      Cellular senescence has become an increasingly important target in the development of novel therapeutics. Emerging data implicates senescence bypass in the development of cancer and suggests that senescence may represent a tumor suppressor mechanism. The demonstration that tumor cells can be induced to undergo replicative senescence following the introduction of negative cell-cycle regulators, anti-telomerase peptides, or drug treatment suggests that induction of senescence can be exploited as a basis for cancer therapy [8,9].

      For Research Use Only; Not for use in diagnostic procedures

      Test Principle:
      As outlined above, a classic characteristic of the senescent phenotype is the induction of senescence-associated β-galactosidase (SA-β-gal) activity. SA-β-gal is present only in senescent cells, not in presenescent, quiescent, or proliferating cells. Chemicon's Cellular Senescence Assay Kit provides all the reagents required to efficiently detect SA-β-gal activity at pH 6.0 in cultured cells and tissue sections. In this assay, SA-β-gal catalyzes the hydrolysis of X-gal, which results in the accumulation of a distinctive blue color in senescent cells. Each kit provides sufficient quantities of reagents to perform at least 50 assays in 35 mm wells.
      Materials Required but Not Delivered1. 1X PBS (Phosphate Buffered Saline)
      2. 37°C incubator
      3. Phase contrast or light microscope
      4. Polypropylene tubes
      5. Cells / Tissue samples
      6. 70% glycerol (optional)
      Product Information
      • 100X Fixing Solution: (Part No. 2004755) One 1.5 mL vial
      • 10X Staining Solution A: (Part No. 2004756) One 15 mL bottle
      • 10X Staining Solution B: (Part No. 2004754) One 15 mL bottle
      • X-gal Solution: (Part No. 2004752) Two 1.5 mL vials
      Detection methodChromogenic
      ApplicationCellular Senescence Assay Kits provide all the reagents required to efficiently detect SA-β-gal activity at pH 6.0 in cultured cells & tissue sections.
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore X-gal solution protected from light at -20°C, and other kit components at 4°C. All components supplied are stable for 1 year.

      Please refer to the Material Safety Data Sheet at www.chemicon.com for any necessary precautions.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 


      Reference overviewPub Med ID
      What has senescence got to do with cancer?
      Dimri, Goberdhan P
      Cancer Cell, 7: 505-12 (2005)  2005

      Show Abstract
      15950900 15950900
      Telomere-independent cellular senescence in human fetal cardiomyocytes
      Ball, Andrew J and Levine, Fred
      Aging Cell, 4(1):21-30 (2005)  2005

      15659210 15659210
      Telomere shortening impairs organ regeneration by inhibiting cell cycle re-entry of a subpopulation of cells
      Satyanarayana, A, et al
      EMBO J, 22(15):4003-13 (2003)  2003

      12881434 12881434
      A senescence program controlled by p53 and p16INK4a contributes to the outcome of cancer therapy
      Schmitt, Clemens A, et al
      Cell, 109(3):335-46 (2002)  2002

      12015983 12015983
      Replicative senescence revisited.
      Marcotte, Richard and Wang, Eugenia
      J. Gerontol. A Biol. Sci. Med. Sci., 57: B257-69 (2002)  2002

      Show Abstract
      12084796 12084796
      Differential expression of thymosin beta-10 by early passage and senescent vascular endothelium is modulated by VPF/VEGF: evidence for senescent endothelial cells in vivo at sites of atherosclerosis
      Vasile, E, et al
      FASEB J, 15(2):458-66 (2001)  2001

      11156961 11156961
      Senescence and immortalization of human cells
      Duncan, E L, et al
      Biogerontology, 1(2):103-21 (2000)  2000

      11707927 11707927
      Expression of senescence-associated beta-galactosidase in enlarged prostates from men with benign prostatic hyperplasia
      Choi, J, et al
      Urology, 56(1):160-6 (2000)  2000

      10869659 10869659
      A biomarker that identifies senescent human cells in culture and in aging skin in vivo
      Dimri, G P, et al
      Proc Natl Acad Sci USA, 92(20):9363-7 (1995)  1995

      7568133 7568133

      Data Sheet

      Cellular Senescence Assay - Data Sheet
      Cellular Senescence Assay - Data Sheet

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      Life Science Research > Cell Analysis > Cell-based Assays > Cell Growth & Transformation Assays