17-10532 | ChIPAb+™ PCAF Antibody

17-10532
25 assays  25 assays per set. Recommended use: 0.7 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HChIP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number17-10532
      Brand Family Upstate
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+™ PCAF Antibody
      OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
      The ChIPAb+ PCAF validated antibody and primer set includes a PCAF antibody, a Normal Rabbit IgG, and positive control primers which amplify 127 bp of human PRAME The PCAF antibody and negative control IgG are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of PCAF associated chromatin.
      Alternate Names
      • K(lysine) acetyltransferase 2B
      • P300/CBP-associated factor
      • Histone acetyltransferase PCAF
      • histone acetyltransferase KAT2B
      • Lysine acetyltransferase 2B
      • Histone acetylase PCAF
      • CREBBP-associated factor
      • P/CAF
      Background InformationP/CAF(sometimes called KAT2B) is a transcription cofactor with a histone acetyltransferase (HAT) activity. Consistent with this protein playing a direct role in transcriptional regulation, P/CAF can acetylate core histones H3 and H4, chromatin proteins HMG17 and HMG I(Y), as well as human immunodeficiency virus Tat, transcription factor MyoD, and general transcription factors TFIIE and TFIIF. A role for P/CAF in cell growth and differentiation is suggested by its interaction in vivo and in vitro with p300/CBP, two large nuclear proteins that bind to sequence-specific factors involved in cell growth/differentiation. A role for P/CAF in cell division is also suggested by data showing that this protein competes with E1A for binding sites in p300/CBP to counteract E1A's mitogenic activity.
      References
      Product Information
      FormatPurified
      Control
      • Includes normal rabbit IgG and primers specific for the human PRAME.
      PresentationAnti-PCAF (Rabbit Polyclonal), Part No. CS216159. One vial containing 17.5 µg purified rabbit polyclonal in 25 µL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of 30% glycerol (0.7 mg/mL final). Store at -20°C.

      Normal Rabbit IgG, Part No. PP64B. One vial containing 125 μg of purified Rabbit IgG in 125 μL storage buffer containing 0.05% sodium azide. Store at -20°C.

      ChIP Primers, PRAME. Part No. CS216150. One vial containing 75 μL of 5 μM of each primer specific for human preferentially expressed antigen in melanoma (chr22:22901678+22901804, hg19 build). Store at -20°C.
      FOR: GAA GCC TCG CCG GAA CTC
      REV: TCC TCC CCA TCT CTG CAG AA
      Applications
      ApplicationThis ChIPAb+ Validated Antibody & Primer Set conveniently includes the antibody, matched IgG negative control antibody & set control PCR primers that detect a known positive locus.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      Application NotesChromatin Immunoprecipitation:
      Sonicated chromatin prepared from K562 cells (~4e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 0.7 µg of either Normal Rabbit IgG (Part No. PP64B), or 0.7 µg Anti-PCAF (Part No. CS216159) and the Magna ChIP™ HiSens (Cat. # 17-10460). Successful immunoprecipitation of PCAF associated DNA fragments was verified by qPCR using ChIP Primers, PRAME (Part No. CS216150) as a positive locus, and ChIP Primers, SNRPN (Part No. CS207347) as a negative locus.
      Please refer to the Magna ChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens(Cat. # 17-10461) protocol for experimental details.

      Chromatin Immunoprecipitation:
      Sonicated chromatin prepared from K562 cells (1e6, 4e6 and 1e7 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 0.7 µg of either Normal Rabbit IgG (Part No. PP64B), or 0.7 µg Anti-PCAF (Part No. CS216159) and the Magna ChIP™ HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of PCAF associated DNA fragments was verified by qPCR using ChIP Primers, PRAME (Part No. CS216150)
      Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details.

      Western Blotting Analysis:
      0.1 µg/mL of this antibody detected PCAF on 5, 15 and 50 µg of HeLa whole cell lysate.
      Biological Information
      ImmunogenRecombinant protein corresponding to human PCAF (a.a. 782-832).
      EpitopeLocated between amino acids 782-832
      HostRabbit
      SpecificityRecognizes PCAF in regions between a.a. 782-832.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with human. May react with additional species due to sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GCN5
      • GCN5L
      • P/CAF
      • P
      • CAF
      • PCAF
      • KAT2B
      UniProt Number
      Molecular Weight~93 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceChromatin Immunoprecipitation:
      Sonicated chromatin prepared from K562 cells fixed for 10 min (4e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 0.7 µg of either Normal Rabbit IgG (Part No. PP64B), or 0.7 µg Anti-PCAF (Part No. CS216159) and the Magna ChIP™ HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of PCAF associated DNA fragments was verified by qPCR using ChIP Primers, PRAME (Part No. CS216150).
      Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 assays
      Material Package25 assays per set. Recommended use: 0.7 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies