SCC036 | Human Mesenchymal Stem Cells (derived from hES cells)

SCC036
1 kit containing 1 million viable cells   
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCC036
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionHuman Mesenchymal Stem Cells (derived from hES cells)
      References
      Product Information
      Applications
      ApplicationIncludes 1 million viable cells of Human Mesenchymal Stem Cells (derived from hES cells).
      Key Applications
      • Stem Cell Culture
      Application NotesMesenchymal stem cells (MSCs) are defined as a self-renewing population of adherent, multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages. In defined in vitro assays, mesenchymal stem cells have been shown to readily differentiate into lineage-specific cells that form bone, cartilage, fat, tendon, and muscle tissue. Mesenchymal stem cells have been isolated from adult bone marrow, adipose tissue dermis, and other connective tissues. Isolation of MSCs from these sources typically requires invasive procedures and availability of a suitable donor. The number of MSCs that can be isolated from a single donor and the capacity for long term proliferation is limited. Mesenchymal stem cells derived from human embryonic stem cells provide an attractive alternative source to a potentially unlimited supply of cells with multilineage differentiation capacity.
      EMD Millipore’s Human Mesenchymal Stem Cells (MSC) are derived from MEL-1 human embryonic stem cells (hESC). The cells proliferate as an adherent cell monolayer and can be expanded for up to 10 passages. Human ESC-derived MSC express the appropriate MSC markers including H-CAM (CD44), STRO-1, Thy-1 (CD90), and M-CAM (CD146) and do not express hematopoietic cell surface markers, CD14 (present on leukocytes) and CD19 (present on B-lymphocytes) and pluripotent markers, Oct-4 and SSEA-4. Cells can readily differentiate into adipocytes and osteoctyes.
      EMD Millipore’s cryopreserved Human Mesenchymal Stem Cells derived from human ESC (Cat. No. SCC036) can be optimally expanded in low-serum media (FibroGRO LS Complete Media Kit for Culture of Human Fibroblasts, Cat. No. SCMF002). For serum-free applications, cells can also be expanded in serum-free media (FibroGRO Complete Media Kit for Culture of Human Fibroblasts, Cat. No. SCMF001).

      Biological Information
      SourceHuman ES Cells (Mel-1 cell line)
      Stem Cell Type
      • Mesenchymal Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEMD Millipore’s Human Mesenchymal Stem Cells are derived from MEL-1 human embryonic stem cells and have been validated for high expression level of cell surface molecules that are present on mesenchymal stem cells: H-CAM (CD44), M-CAM (CD146), THY-1 (CD90), and STRO-1 and for their absence of hematopoietic and pluripotent cell surface markers, CD14 (present on leukocytes), CD19 (present on B-lymphocytes) and Oct-4 and SSEA-4 (present on undifferentiated human ES cells), respectively. The cells have also been validated for their self-renewal and multi-lineage differentiation capacities (please refer to product manual figures for representative data). Cells display normal karyotype as assessed by G-banding of 20 metaphase cells and tested negative for mycoplasma.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsHuman Mesenchymal Stem Cells(derived from hES cells) (Part # SCC036) should be stored in liquid nitrogen. It is recommended that the cells be used within ten passages.
      M
      Packaging Information
      Material Size1 kit containing 1 million viable cells
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Accessories

      Catalogue Number Description  
      SCM015 Mesenchymal Stem Cell Expansion Medium (1x) Show Pricing & Availability
      SCMF002 FibroGRO™-LS Complete Media Kit for Culture of Human Fibroblasts Show Pricing & Availability
      SCR067 Human Mesenchymal Stem Cell Characterization Kit Show Pricing & Availability
      SCR108 Human Mesenchymal Stem Cell Kit - Derived from Bone-Marrow Show Pricing & Availability

      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > Stem Cell Lines