GF342 | LIF,
Human Recombinant Animal Free

GF342
25µg  
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      Overview

      Replacement Information

      Key Spec Table

      SourcePurity
      Protein is expressed in E.coli.
      The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.
      Product endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
      Description
      Catalogue NumberGF342
      DescriptionLIF,
      Human Recombinant Animal Free
      Alternate Names
      • Leukemia Inhibitory Factor
      • Emfilermin
      • MLPLI
      • HILDA
      • D-FACTOR
      Background InformationLIF is a pleiotrophic factor produced by multiple cell types including T cells, myelomoncytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor.
      Recombinant human LIF is a 19.6 kDa protein containing 180 amino acids residues including three disulfide bonds.
      References
      Product Information
      PresentationProduct is presented without additives and is filtered through a 0.2 micron filter before lyophilization.
      Applications
      Biological Information
      PurityProduct endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
      SourceProtein is expressed in E.coli.
      The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceDetermined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤0.1 ng/ml, corresponding to a specific activity of ≥1 x 10^7 units/mg.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
      Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.
      Packaging Information
      Material Size25µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      LIF, Human Recombinant Animal Free - 2468399 2468399
      LIF, Human Recombinant Animal Free - Q2436473 Q2436473
      LIF, -2644001 2644001
      LIF, -2714456 2714456
      LIF, -2728266 2728266
      LIF, Human Recombinant Animal Free -2489232 2489232
      LIF, Human Recombinant Animal Free -2626054 2626054
      LIF, Human Recombinant Animal Free -2745939 2745939
      LIF,Human Recombinant Animal Free -2596679 2596679
      LIF,Human Recombinant Animal Free -2619741 2619741

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors