14-429M | MEK1 Protein, active, 250 µg

14-429M
250 µg  Also available in 10 μg size (cat#14-429) and in bulk (cat# 14-429-K).
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information
      Description
      Catalogue Number14-429M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMEK1 Protein, active, 250 µg
      OverviewN-terminal GST and Cterminal His6-tagged, recombinant full-length human MEK1
      References
      Product Information
      Applications
      ApplicationActive, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed in E. coli
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
      Gene Symbol
      • MAP2K1
      • MAPKK1
      • PRKMK1
      • MEK1
      • MKK1
      Protein TargetMEK1
      Purification MethodPurified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose
      Target Sub-FamilySTE
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
      SIZE: 393 amino acids; 43439 Da
      SUBUNIT: Interacts with MORG1 (By similarity). Interacts with Yersinia yopJ.
      PTM: Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. & Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
      DISEASE: SwissProt: Q02750 # Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
      SIMILARITY: SwissProt: Q02750 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight71kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of MBP
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-429) and in bulk (cat# 14-429-K).
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-429 MEK1 Protein, active, 10 µg Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases