HNABTMAG-68K | MILLIPLEX MAP Human Amyloid Beta and Tau Magnetic Bead Panel - Multiplex Assay

HNABTMAG-68K
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Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Tau (Total), Amyloid β40, Amyloid β42, Tau (pThr181)HMplexLuminex xMAP
Description
Catalogue NumberHNABTMAG-68K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Amyloid Beta and Tau Magnetic Bead Panel - Multiplex Assay
OverviewThe MILLIPLEX® MAP Human Amyloid Beta Tau Magnetic BeadPanel is to be used for the simultaneous quantification of Aβ40, Aβ42, tTau, and pTau181. This kit may be used only for the analysis of cerebrospinal fluid (CSF). This is a configurable assay, and some or all analytes can be selected: tau (total), amyloid β40, amyloid β42 and tau (pThr181).

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® MAP panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance

• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

• Optimized serum matrix to mimic native analyte environment

• Detection antibody cocktails designed to yield consistent analyte profiles within panel


EMD Millipore’s MILLIPLEX® MAP Human Amyloid Beta Tau Magnetic Bead Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background InformationThe MILLIPLEX® MAP Human Amyloid Beta and Tau Panel enables you to focus on the relevance and therapeutic potential of Aβ40, Aβ42, Total Tau proteins (tTau), and Phosphorylated Tau Thr181 (pTau181) in your research model. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.
References
Product Information
Components
  • Human ABeta and Tau Standard, 1 vial, lyophilized
  • Human ABeta and Tau Quality Control 1, 1 vial, lyophilized
  • Human ABeta and Tau Quality Control 2, 1 vial, lyophilized
  • Bead Diluent, 1 bottle, 3.5mL
  • Set of one 96-Well Plate with 2 sealers
  • Assay Buffer, 1 bottle, 30 mL
  • Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
  • Human ABeta and Tau Detection Antibodies, 1 bottle, 3.2 mL
  • Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
  • Mixing Bottle, 1 bottle
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 15% CV
  • Intra-assay: < 10% CV
Panel TypeMAGNETIC Neuroscience
Applications
ApplicationThe MILLIPLEX® MAP Human Amyloid Beta and Tau Panel is a 4-plex kit containing all reagents needed for simultaneous quantification of Aβ40, Aβ42, Total Tau proteins (tTau), and Phosphorylated Tau Thr181 (pTau181) in CSF samples.
Key Applications
  • Multiplexing
Application NotesEMD Millipore’s MILLIPLEX® MAP Human Amyloid Beta and Tau Panel is a 4-plex kit used for the simultaneous quantification of any or all of the following analytes in cerebrospinal fluid (CSF) samples: Amyloid beta 1-40 (Aβ40), Amyloid beta 1-42 (Aβ42), Total Tau proteins (tTau), and Phosphorylated Tau Thr181 (pTau181).
• This is a competitive assay. An overnight (16-18 hours) incubation at 20-25°C is recommended.
• This assay requires 25 μL of diluted cerebrospinal fluid (CSF).
• A 1:2 sample dilution is recommended for extracted CSF samples.
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity
  • Human
Analytes Available
  • Tau (Total)
  • Amyloid β40
  • Amyloid β42
  • Tau (pThr181)
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Accuracy
  • Spike recovery for Aβ1-40 is 101%.
  • Spike recovery for Aβ1-42 is 109%.
  • Spike recovery for tTau is 98%.
  • Spike recovery for pTau181 is 91%
Physicochemical Information
Sensitivity
  • Aβ1-40: 10.2 pg/mL (MinDC+2SD)
  • Aβ1-42: 2.5 pg/mL (MinDC+2SD)
  • tTau: 14.2 pg/mL (MinDC+2SD)
  • pTau181: 1.5 pg/mL (MinDC+2SD)
Standard Curve Range
  • Aβ1-40: 21-15000 pg/mL
  • Aβ1-42: 2-2000 pg/mL
  • tTau: 11-8000 pg/mL
  • pTau181: 0.7-500 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Human Amyloid Beta and Tau Magnetic Bead Panel

SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Custom Immunoassay Menu of Services

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Application Note: Multiplex detection of four Alzheimer’s Disease biomarkers in human CSF using a novel MILLIPLEX® assay panel
Quality Control Ranges: MILLIPLEX® MAP Human Amyloid Beta and Tau Magnetic Bead Panel

Posters

Title
MILLIPLEX MAP: Exercise, Signaling, and Your Brain
Multiplex Assays of Fifteen Human Complement Factors

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Categories

Life Science Research > Protein Detection and Quantification > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Configurable Kits
Life Science Research > Antibodies and Assays > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Configurable Kits